Abstract：
Members of the Rho family of small monomeric GTPases regulate a plethora of critical cellular functions including gene expression, cell cycle progression, and the dynamic modeling of the actin cytoskeleton. Diversity among Rho family members is derived, in part, from variations in their subcellular distribution. Localization of newly synthesized (naïve) Rho proteins to target subcellular compartments is largely governed by lipid modifications, including posttranslational prenylation. Here, using well-established and widely available contemporary methodologies, detailed protocols by which to semiquantitatively evaluate the functional consequence of posttranslational prenylation in human trabecular meshwork cells are described. We propose the novel concept that posttranslational prenylation itself is a key regulator of mammalian Rho GTPase protein expression and turnover.
摘要：
小单体GTP酶的Rho家族成员调节大量关键细胞功能，包括基因表达，细胞周期进程，以及肌动蛋白细胞骨架的动态建模。衍生出Rho家族成员之间的多样性，在某种程度上,来自它们亚细胞分布的变化。新合成的（幼稚）Rho蛋白在靶向亚细胞区室中的定位在很大程度上受到脂质修饰的控制。包括翻译后异戊二烯化。这里,使用成熟和广泛可用的当代方法，描述了半定量评估人小梁细胞中翻译后异戊二烯化的功能后果的详细方案。我们提出了一个新概念，即翻译后戊烯化本身是哺乳动物RhoGTP酶蛋白表达和周转的关键调节因子。
