Abstract：
As 3D culture technology advances, new avenues have opened for the development of physiological human cancer models. These preclinical models provide efficient ways to translate basic cancer research into clinical tumor therapies. Recently, cancer organoids have emerged as a model to dissect the more complex tumor microenvironment. Incorporating cancer organoids into preclinical programs have the potential to increase the success rate of oncology drug development and recapitulate the most efficacious treatment regimens for cancer patients. In this review, four main types of cancer organoids are introduced, their applications, advantages, limitations, and prospects are discussed, as well as the recent application of single-cell RNA-sequencing (scRNA-seq) in exploring cancer organoids to advance this field.
摘要：
随着3D文化技术的进步，人类生理性癌症模型的发展开辟了新的途径。这些临床前模型提供了将基础癌症研究转化为临床肿瘤治疗的有效方法。最近,癌症类器官已成为解剖更复杂的肿瘤微环境的模型。将癌症类器官纳入临床前计划有可能提高肿瘤药物开发的成功率，并为癌症患者概述最有效的治疗方案。在这次审查中,介绍了四种主要类型的癌症器官，他们的应用,优势,局限性,并讨论了前景，以及最近单细胞RNA测序（scRNA-seq）在探索癌症类器官以推进这一领域的应用。
