METHODS: A prospective case-control tertiary-center study included 46 women aged 22-45 undergoing laparoscopy due to pelvic endometriosis (n = 32; arm 1) and idiopathic infertility (n = 14; arm 2). A sample of the greater omentum was collected intraoperatively for detection of 16 standard PAHs by gas chromatography-isotope dilution mass spectrometry method. PAHs concentrations were compared in both study arms. The associations between PAHs concentrations and selected variables were investigated.
RESULTS: There were no significant differences between both arms in terms of reference PAHs concentrations, nor correlations between PAHs concentrations and the stage of endometriosis. However, notable differences were observed in specific PAHs concentrations related to certain conditions. The concentrations of acenaphthene (p = 0.016) and fluorene (p = 0.013) were significantly lower in women with peritoneal adhesions, while the concentrations of benz[a]anthracene, benzo[k]fluoranthene and indeno[1,2,3-cd]pyrene [ng/g] were higher in cigarette smokers.
CONCLUSIONS: The study showed no differences in exposure to PAHs between women with and without pelvic endometriosis. Determining the toxicity of PAHs in endometriosis requires further research.
方法:一项前瞻性病例对照三级中心研究包括46名22-45岁因盆腔子宫内膜异位症(n=32;第1组)和特发性不孕症(n=14;第2组)接受腹腔镜检查的妇女。术中收集大网膜样品,通过气相色谱-同位素稀释质谱法检测16种标准PAHs。在两个研究组中比较PAHs浓度。研究了PAHs浓度与选定变量之间的关联。
结果:在参考PAHs浓度方面,两组之间没有显着差异,PAHs浓度与子宫内膜异位症分期之间也没有相关性。然而,在与某些条件相关的特定PAHs浓度中观察到显着差异。在有腹膜粘连的妇女中,emenaphthene(p=0.016)和芴(p=0.013)的浓度显着降低,而苯并[a]蒽的浓度,吸烟者的苯并[k]荧蒽和茚并[1,2,3-cd]芘[ng/g]较高。
结论:研究表明,有和没有盆腔子宫内膜异位症的女性在PAHs暴露方面没有差异。确定子宫内膜异位症中PAHs的毒性需要进一步研究。