Abstract：
Copper (Cu) is a crucial element that plays a vital role in facilitating proper biological activities in living organisms. In this study, copper oxide nanoparticles (CuO NPs) were synthesized using a straightforward precipitation chemical method from a copper nitrate precursor at a temperature of 85 °C. Subsequently, these NPs were coated with the aqueous extract of Sargassum angustifolium algae. The size, morphology, and coating of the NPs were analyzed through various methods, revealing dimensions of approximately 50 nm, a multidimensional shaped structure, and successful algae coating. The antibacterial activity of both coated and uncoated CuO NPs against Vibrio harveyi, a significant pathogen in Litopenaeus vannamei, was investigated. Results indicated that the minimum inhibitory concentration (MIC) for uncoated CuO NPs was 1000 μg/mL, whereas for coated CuO NPs, it was 500 μg/mL. Moreover, the antioxidant activity of the synthesized NPs was assessed. Interestingly, uncoated CuO NPs exhibited superior antioxidant activity (IC50 ≥ 16 μg/mL). The study also explored the cytotoxicity of different concentrations (10-100 μg/mL) of both coated and uncoated CuO NPs. Following 48 h of incubation, cell viability assays on shrimp hemocytes and human lymphocytes were conducted. The findings indicated that CuO NPs coated with alga extract at a concentration of 10 μg/mL increased shrimp hemocyte viability. In contrast, uncoated CuO NPs at a concentration of 25 μg/mL and higher, as well as CuO NPs at a concentration of 50 μg/mL and higher, led to a decrease in shrimp hemocyte survival. Notably, this study represents the first quantitative assessment of the toxicity of CuO NPs on shrimp cells, allowing for a comparative analysis with human cells.
摘要：
铜（Cu）是一种至关重要的元素，在促进生物体的适当生物活动中起着至关重要的作用。在这项研究中,氧化铜纳米颗粒(CuONPs)使用简单的沉淀化学方法从硝酸铜前体在85°C的温度下合成。随后，这些NP用马尾藻藻的水提取物包被。大小,形态学,并通过各种方法分析了NPs的涂层，揭示大约50纳米的尺寸，多维形状的结构，和成功的藻类涂层。涂层和未涂层的CuONPs对哈氏弧菌的抗菌活性，凡纳滨对虾是一种重要的病原体，被调查。结果表明，未包被的CuONPs的最小抑制浓度（MIC）为1,000μg/mL，而对于涂覆的CuONPs，它是500μg/mL。此外，评估合成的NP的抗氧化活性。有趣的是,未包被的CuONPs表现出优异的抗氧化活性(IC50≥16μg/mL)。该研究还探索了不同浓度(10-100μg/mL)的涂覆和未涂覆的CuONP的细胞毒性。孵育48小时后，对虾血细胞和人淋巴细胞进行细胞活力测定。结果表明，浓度为10μg/mL的藻类提取物包被的CuONPs增加了虾血细胞的活力。相比之下,浓度为25μg/mL或更高的未涂覆的CuONPs，以及浓度为50μg/mL或更高的CuONPs，导致虾血细胞存活率下降。值得注意的是,这项研究代表了CuONPs对虾细胞毒性的首次定量评估，允许与人类细胞进行比较分析。
