Abstract：
The polysaccharide glucan was extracted from Gastrodia elata Blume, and its structural characterizations and beneficial effects against acute dextran sulfate sodium (DSS)-induced ulcerative colitis were investigated. The results showed that a polysaccharide GP with a molecular weight of 811.0 kDa was isolated from G. elata Blume. It had a backbone of α-D-1,4-linked glucan with branches of α-d-glucose linked to the C-6 position. GP exhibited protective effects against DSS-induced ulcerative colitis, and reflected in ameliorating weight loss and pathological damages in mice, increasing colon length, inhibiting the expression of inflammatory cytokines tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β), decreasing the levels of inflammatory related proteins NLRP3 and ASC, and elevating the anti-inflammatory cytokine interleukin-10 (IL-10) level in mouse colon tissues. GP supplementation also reinforced the intestinal barrier by promoting the expression of ZO-1, Occludin, and MUC2 of colon tissues, and positively regulated intestinal microbiota. Thus, GP treatment possessed a significant improvement in ulcerative colitis in mice, and it was expected to be developed as a functional food.
摘要：
从天麻中提取多糖葡聚糖，研究了其结构特征和对急性葡聚糖硫酸钠（DSS）诱导的溃疡性结肠炎的有益作用。结果表明，从G.elatablume中分离出分子量为811.0kDa的多糖GP。其具有α-D-1,4-连接的葡聚糖的主链,具有连接至C-6位置的α-d-葡萄糖的分支。GP对DSS诱导的溃疡性结肠炎表现出保护作用，并反映在改善小鼠的体重减轻和病理损伤上，增加结肠长度，抑制炎性细胞因子肿瘤坏死因子-α(TNF-α)和白细胞介素-1β(IL-1β)的表达,降低炎症相关蛋白NLRP3和ASC的水平，并提高小鼠结肠组织中的抗炎细胞因子白细胞介素-10(IL-10)水平。GP补充还通过促进ZO-1，Occludin的表达来增强肠屏障，和结肠组织的MUC2，和积极调节肠道微生物群。因此，GP治疗对小鼠溃疡性结肠炎有显著改善，它被认为是一种功能性食品。
