PDF(Pubmed)
Abstract：
BACKGROUND: Endodontic treatment is one of the main dental treatments to manage inflamed or infected root canal systems of teeth. The success of endodontic treatment principally depends on eradicating microorganisms in the root canal by chemo-mechanical debridement with irrigation solutions like sodium hypochlorite (NaOCl). NaOCl has been used in concentrations ranging from 0.5% to 5.25%. This study determined the antimicrobial effectiveness of selected concentrations (0.5%, 1.0%, 2.6%, and 5.2%) of NaOCl in endodontic treatment.
METHODS: The study sites were the University of Ghana Dental School (UGDS) and Noguchi Memorial Institute for Medical Research (NMIMR). Sixty infected single-rooted single-canal teeth were used. Before (S1) and after (S2), root canal samples during the endodontic treatment with the selected concentrations of NaOCl were examined via anaerobic and aerobic cultures. The isolates were identified using Matrix Assisted Laser Desorption Ionization-Time Of Flight Mass Spectrometry (MALDI-TOF MS).
RESULTS: All S1 samples were positive for cultivable bacteria. Fifty-three (53) different microbial species belonging to 20 different microbial genera were isolated. Streptococcus viridans was the most frequently isolated microbe. There were zero isolates in the root canals irrigated with 2.6% and 5.2% NaOCl. Two teeth had isolates in the groups irrigated with the lower concentrations (0.5% and 1.0%) of NaOCl. The persistent bacteria were one species each of Streptococcus mitis and Streptococcus oralis, respectively.
CONCLUSIONS: Root canal treatments using chemo-mechanical preparation with the selected concentrations (0.5%, 1.0%, 2.6%, and 5.2%) of NaOCl were effective in significantly reducing the microbial load, and for the 5.2% and 2.6% concentrations, in eliminating all the microorganisms.
METHODS: The study sites were the University of Ghana Dental School (UGDS) and Noguchi Memorial Institute for Medical Research (NMIMR). Sixty infected single-rooted single-canal teeth were used. Before (S1) and after (S2), root canal samples during the endodontic treatment with the selected concentrations of NaOCl were examined via anaerobic and aerobic cultures. The isolates were identified using Matrix Assisted Laser Desorption Ionization-Time Of Flight Mass Spectrometry (MALDI-TOF MS).
RESULTS: All S1 samples were positive for cultivable bacteria. Fifty-three (53) different microbial species belonging to 20 different microbial genera were isolated. Streptococcus viridans was the most frequently isolated microbe. There were zero isolates in the root canals irrigated with 2.6% and 5.2% NaOCl. Two teeth had isolates in the groups irrigated with the lower concentrations (0.5% and 1.0%) of NaOCl. The persistent bacteria were one species each of Streptococcus mitis and Streptococcus oralis, respectively.
CONCLUSIONS: Root canal treatments using chemo-mechanical preparation with the selected concentrations (0.5%, 1.0%, 2.6%, and 5.2%) of NaOCl were effective in significantly reducing the microbial load, and for the 5.2% and 2.6% concentrations, in eliminating all the microorganisms.
摘要：
背景：牙髓治疗是治疗发炎或感染的牙齿根管系统的主要牙科治疗方法之一。牙髓治疗的成功主要取决于通过使用次氯酸钠（NaOCl）等冲洗溶液进行化学机械清创来根除根管中的微生物。NaOCl的使用浓度范围为0.5%至5.25%。本研究确定了选定浓度(0.5%，1.0%，2.6%,和5.2％）的NaOCl在牙髓治疗中。
方法：研究地点是加纳大学牙科学院（UGDS）和野口纪念医学研究所（NMIMR）。使用了60颗感染的单根单管牙齿。之前（S1）和之后（S2），通过厌氧和需氧培养物检查用选定浓度的NaOCl进行牙髓治疗期间的根管样品。使用基质辅助激光解吸电离-飞行时间质谱(MALDI-TOFMS)鉴定分离物。
结果：所有S1样品对可培养细菌呈阳性。分离了属于20个不同微生物属的53个不同微生物物种。草绿色链球菌是最常见的分离微生物。用2.6％和5.2％的NaOCl灌溉的根管中没有分离物。在用较低浓度（0.5％和1.0％）的NaOCl灌溉的组中，有两颗牙齿具有分离物。持久性细菌是链球菌和口腔链球菌各一种，分别。
结论：使用选定浓度的化学机械制剂进行根管治疗（0.5％，1.0%，2.6%,5.2%)的NaOCl能显著降低微生物负荷，对于5.2%和2.6%的浓度，消除所有的微生物。
方法：研究地点是加纳大学牙科学院（UGDS）和野口纪念医学研究所（NMIMR）。使用了60颗感染的单根单管牙齿。之前（S1）和之后（S2），通过厌氧和需氧培养物检查用选定浓度的NaOCl进行牙髓治疗期间的根管样品。使用基质辅助激光解吸电离-飞行时间质谱(MALDI-TOFMS)鉴定分离物。
结果：所有S1样品对可培养细菌呈阳性。分离了属于20个不同微生物属的53个不同微生物物种。草绿色链球菌是最常见的分离微生物。用2.6％和5.2％的NaOCl灌溉的根管中没有分离物。在用较低浓度（0.5％和1.0％）的NaOCl灌溉的组中，有两颗牙齿具有分离物。持久性细菌是链球菌和口腔链球菌各一种，分别。
结论：使用选定浓度的化学机械制剂进行根管治疗（0.5％，1.0%，2.6%,5.2%)的NaOCl能显著降低微生物负荷，对于5.2%和2.6%的浓度，消除所有的微生物。
