Abstract：
Ubiquitin-fold modifier 1 (UFM1) is attached to protein substrates through the sequential activity of an E1 (UBA5)-E2 (UFC1)-E3 (UFL1) cascade. UFL1 is the E3 ligase for UFMylation in vertebrates. However, there have been no studies on UFL1 in silkworm to date. In this study, we identified a UFL1 ortholog in Bombyx mori genome. Spatio-temporal expression profiles showed that BmUFL1 expression was high in the midgut, epidermis, and testis and in the pupa-adult stage. BmUFL1 knockdown inhibited B. mori nucleopolyhedrovirus (BmNPV) proliferation, while BmUFL1 overexpression promoted BmNPV proliferation. Mechanically, protein kinase RNA-like endoplasmic reticulum kinase (PERK) signaling and cell apoptosis are involved in BmUFL1-regulated BmNPV proliferation. Overall, these results suggest that BmUFL1 facilitates BmNPV proliferation in silkworm.
摘要：
泛素-折叠修饰剂1(UFM1)通过E1(UBA5)-E2(UFC1)-E3(UFL1)级联的顺序活性附着于蛋白质底物。UFL1是脊椎动物中UFM化的E3连接酶。然而,迄今为止，还没有关于家蚕UFL1的研究。在这项研究中,我们在家蚕基因组中鉴定了一个UFL1直系同源物。时空表达谱显示，BmUFL1在中肠中表达较高，表皮,和睾丸，在蛹成虫阶段。BmUFL1基因敲除抑制了桑树核型多角体病毒(BmNPV)的增殖,而BmUFL1过表达促进BmNPV增殖。机械上,蛋白激酶RNA样内质网激酶（PERK）信号传导和细胞凋亡参与BmUFL1调节的BmNPV增殖。总的来说,这些结果表明BmUFL1促进家蚕BmNPV的增殖。
