Abstract：
The main objective of this study was to investigate the potential efficacy of carvacrol (CAR) in mitigating bleomycin (BLM)-induced pulmonary fibrosis (PF). Sixty-six male Wistar rats were assigned into two main groups of 7 and 21 days. They were divided into the subgroups of control, BLM, CAR 80 (only for the 21-day group), and CAR treatment groups. The CAR treatment groups received CAR (20, 40, and 80 mg/kg, orally) for 7 or 21 days after an instillation of BLM (5 mg/kg, intratracheally). Results indicated that BLM significantly increased total cell count in bronchoalveolar lavage fluid and the percentages of neutrophils and lymphocytes, and reduced the percentage of macrophages. CAR dose-dependently decreased total cell count and the percentage of neutrophils and lymphocytes. CAR significantly reduced thiobarbituric acid reactive substances and hydroxyproline levels and elevated the total thiol level and catalase, superoxide dismutase, and glutathione peroxidase activities in BLM-exposed rats. Furthermore, CAR decreased the transforming growth factor-β1, connective transforming growth factor, and tumor necrosis factor-α on days 7 and 21. BLM increased interferon-γ on day 7 but decreased its level on day 21. However, CAR reversed interferon-γ levels on days 7 and 21. Based on histopathological findings, BLM induced inflammation on days 7 and 21, but for induction of fibrosis, 21-day study showed more fibrotic injuries than the 7-day group. CAR showed the improvement of fibrotic injuries. The effect of CAR against BLM-induced pulmonary fibrosis is possibly due to its antioxidant, anti-inflammatory, and antifibrotic activity.
摘要：
这项研究的主要目的是研究香芹酚（CAR）在减轻博来霉素（BLM）诱导的肺纤维化（PF）中的潜在功效。将66只雄性Wistar大鼠分为7天和21天的两个主要组。他们被分为对照组，BLM,CAR80（仅用于21天的组），和CAR治疗组。CAR治疗组接受CAR（20、40和80mg/kg，口服）滴注BLM（5mg/kg，气管内）。结果表明，BLM显着增加支气管肺泡灌洗液中的总细胞计数以及中性粒细胞和淋巴细胞的百分比，减少了巨噬细胞的比例.CAR剂量依赖性地降低总细胞计数以及中性粒细胞和淋巴细胞的百分比。CAR显着降低硫代巴比妥酸反应性物质和羟脯氨酸水平，并提高总硫醇水平和过氧化氢酶，超氧化物歧化酶,和BLM暴露大鼠的谷胱甘肽过氧化物酶活性。此外，CAR降低了转化生长因子-β1，结缔组织转化生长因子，和肿瘤坏死因子-α在第7天和第21天。BLM在第7天增加干扰素-γ,但在第21天降低其水平。然而,CAR在第7天和第21天逆转了干扰素-γ水平。根据组织病理学发现,BLM在第7天和第21天诱导炎症，但对于诱导纤维化，21天的研究显示比7天组更多的纤维化损伤。CAR显示纤维化损伤的改善。CAR对BLM诱导的肺纤维化的作用可能是由于其抗氧化剂，抗炎,和抗纤维化活性。
