Abstract：
The protein-protein interaction (PPI) network of the Mediator complex is very tightly regulated and depends on different developmental and environmental cues. Here, we present an interactive platform for comparative analysis of the Mediator subunits from humans, baker\'s yeast Saccharomyces cerevisiae, and model plant Arabidopsis thaliana in a user-friendly web-interface database called MediatorWeb. MediatorWeb provides an interface to visualize and analyze the PPI network of Mediator subunits. The database facilitates downloading the untargeted and unweighted network of Mediator complex, its submodules, and individual Mediator subunits to better visualize the importance of individual Mediator subunits or their submodules. Further, MediatorWeb offers network visualization of the Mediator complex and interacting proteins that are functionally annotated. This feature provides clues to understand functions of Mediator subunits in different processes. In an additional tab, MediatorWeb provides quick access to secondary and tertiary structures, as well as residue-level contact information for Mediator subunits in each of the three model organisms. Another useful feature of MediatorWeb is detection of interologs based on orthologous analyses, which can provide clues to understand the functions of Mediator complex in less explored kingdoms. Thus, MediatorWeb and its features can help the user to understand the role of Mediator complex and its subunits in the transcription regulation of gene expression.
摘要：
介体复合物的蛋白质-蛋白质相互作用（PPI）网络受到非常严格的调节，并取决于不同的发育和环境线索。这里,我们提供了一个交互式平台，用于比较分析来自人类的Mediator亚基，面包酵母酿酒酵母，并在称为MediatorWeb的用户友好的Web界面数据库中建立拟南芥模型。MediatorWeb提供了可视化和分析Mediator子单元的PPI网络的界面。该数据库便于下载中介复杂的非目标和未加权网络，它的子模块，和各个Mediator子单元，以更好地可视化各个Mediator子单元或其子模块的重要性。Further,MediatorWeb提供了Mediator复合物和功能注释的相互作用蛋白质的网络可视化。此功能为了解不同进程中Mediator子单元的功能提供了线索。在其他选项卡中，MediatorWeb提供了对二级和三级结构的快速访问，以及三种模式生物中介体亚基的残基级联系信息。MediatorWeb的另一个有用的功能是基于直系同源分析的互系生物检测，这可以提供线索来理解中介复合体在较少探索的王国中的功能。因此，MediatorWeb及其功能可以帮助用户了解Mediator复合物及其亚基在基因表达转录调控中的作用。
