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Abstract：
Although members of the genus Pseudomonas share specific morphological, metabolic, and genomic traits, the diversity of niches and lifestyles adopted by the family members is vast. One species of the group, Pseudomonas putida, thrives as a colonizer of plant roots and frequently inhabits soils polluted with various types of chemical waste. Owing to a combination of historical contingencies and inherent qualities, a particular strain, P. putida KT2440, emerged time ago as an archetype of an environmental microorganism amenable to recombinant DNA technologies, which was also capable of catabolizing chemical pollutants. Later, the same bacterium progressed as a reliable platform for programming traits and activities in various biotechnological applications. This article summarizes the stepwise upgrading of P. putida KT2440 from being a system for fundamental studies on the biodegradation of aromatic compounds (especially when harboring the TOL plasmid pWW0) to its adoption as a chassis of choice in metabolic engineering and synthetic biology. Although there are remaining uncertainties about the taxonomic classification of KT2440, advanced genome editing capabilities allow us to tailor its genetic makeup to meet specific needs. This makes its traditional categorization somewhat less important, while also increasing the strain\'s overall value for contemporary industrial and environmental uses.
摘要：
尽管假单胞菌属的成员具有特定的形态，新陈代谢，和基因组特征，家庭成员采用的利基和生活方式的多样性是巨大的。该组的一个物种，恶臭假单胞菌,作为植物根部的定殖者而茁壮成长，并经常栖息在被各种类型的化学废物污染的土壤中。由于历史偶然性和内在素质的结合，一种特殊的菌株，P.putidaKT2440，作为一种适合重组DNA技术的环境微生物的原型出现，它也能够分解化学污染物。稍后，同样的细菌发展成为在各种生物技术应用中编程特性和活动的可靠平台。本文总结了P.putidaKT2440的逐步升级，从作为芳香族化合物生物降解的基础研究系统（尤其是当携带TOL质粒pWW0时）到将其用作代谢工程和合成生物学的选择基础。尽管KT2440的分类学分类仍存在不确定性，但先进的基因组编辑功能使我们能够调整其遗传组成以满足特定需求。这使得它的传统分类不太重要，同时也增加了菌株对当代工业和环境用途的整体价值。
