关键词: marmoset metabolome metagenomics microbiome nonhuman microbiome nonhuman microbiota nutrition physiology primate veterinary microbiology

来  源:   DOI:10.1128/msystems.00108-24

Abstract:
Gastrointestinal diseases are the most frequently reported clinical problems in captive common marmosets (Callithrix jacchus), often affecting the health and welfare of the animal and ultimately their use as a research subject. The microbiome has been shown to be intimately connected to diet and gastrointestinal health. Here, we use shotgun metagenomics and untargeted metabolomics in fecal samples of common marmosets collected before, during, and after a dietary transition from a biscuit to a gel diet. The overall health of marmosets, measured as weight recovery and reproductive outcome, improved after the diet transition. Moreover, each marmoset pair had significant shifts in the microbiome and metabolome after the diet transition. In general, we saw a decrease in Escherichia coli and Prevotella species and an increase in Bifidobacterium species. Untargeted metabolic profiles indicated that polyamine levels, specifically cadaverine and putrescine, were high after diet transition, suggesting either an increase in excretion or a decrease in intestinal reabsorption at the intestinal level. In conclusion, our data suggest that Bifidobacterium species could potentially be useful as probiotic supplements to the laboratory marmoset diet. Future studies with a larger sample size will be beneficial to show that this is consistent with the diet change.
OBJECTIVE: Appropriate diet and health of the common marmoset in captivity are essential both for the welfare of the animal and to improve experimental outcomes. Our study shows that a gel diet compared to a biscuit diet improves the health of a marmoset colony, is linked to increases in Bifidobacterium species, and increases the removal of molecules associated with disease. The diet transition had an influence on the molecular changes at both the pair and time point group levels, but only at the pair level for the microbial changes. It appears to be more important which genes and functions present changed rather than specific microbes. Further studies are needed to identify specific components that should be considered when choosing an appropriate diet and additional supplementary foods, as well as to validate the benefits of providing probiotics. Probiotics containing Bifidobacterium species appear to be useful as probiotic supplements to the laboratory marmoset diet, but additional work is needed to validate these findings.
摘要:
胃肠道疾病是圈养的普通猴(Callithrixjacchus)中最常见的临床问题,通常会影响动物的健康和福利,并最终将其用作研究对象。微生物组已经被证明与饮食和胃肠道健康密切相关。这里,我们使用鸟枪宏基因组学和非靶向代谢组学对之前收集的普通猕猴的粪便样本,during,在从饼干到凝胶饮食的饮食过渡之后。猴猴的整体健康状况,以体重恢复和生殖结果衡量,饮食过渡后有所改善。此外,在饮食转变后,每对猕猴桃的微生物组和代谢组均发生显著变化.总的来说,我们看到大肠杆菌和普雷沃氏菌种类减少,双歧杆菌种类增加。非目标代谢谱表明多胺水平,特别是尸胺和腐胺,在饮食过渡后很高,表明在肠道水平上排泄增加或肠道重吸收减少。总之,我们的数据表明,双歧杆菌属可能作为益生菌补充剂用于实验室猕猴饮食.具有更大样本量的未来研究将有利于表明这与饮食变化一致。
目的:圈养的普通猴的适当饮食和健康对于动物的福利和改善实验结果都是必不可少的。我们的研究表明,与饼干饮食相比,凝胶饮食可以改善the猴殖民地的健康状况,与双歧杆菌种类的增加有关,并增加与疾病相关分子的去除。饮食转变对配对和时间点群体水平的分子变化都有影响,但只在微生物变化的配对水平上。似乎更重要的是,哪些基因和功能发生了变化,而不是特定的微生物。需要进一步的研究来确定在选择适当的饮食和额外的补充食物时应考虑的特定成分,以及验证提供益生菌的好处。含有双歧杆菌的益生菌似乎可用作实验室猕猴饮食的益生菌补充剂,但需要额外的工作来验证这些发现。
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