Abstract：
Stress has been linked to the development of irritable bowel syndrome (IBS), and various methods have been explored to model IBS in combination with other stimuli. However, it remains unclear whether stress alone can induce IBS in animals. This study aimed to investigate the impact of chronic unpredictable mild stress (CUMS) on gastrointestinal sensation and function in mice and assess the potential of CUMS as a modeling approach for IBS. To evaluate the mice\'s behavior, we conducted open field test, sucrose preference test and weighed the mice, revealing that CUMS indeed induced anxiety and depression in the mice and caused weight loss. Further analyses, including fecal analysis, a total gastrointestinal transport test, and a colon propulsion test, demonstrated that CUMS led to abnormal defecation and disruptions in gastrointestinal motility in the mice. Additionally, the abdominal withdrawal reflex test indicated an increase in visceral sensitivity in CUMS-exposed mice. Histological examination using hematoxylin and eosin staining revealed no significant histological alterations in the colons of CUMS-exposed mice, but it did show a minor degree of inflammatory cell infiltration. In summary, the findings suggest that CUMS can replicate IBS-like symptoms in mice, offering a novel top-down approach to modeling IBS.
摘要：
压力与肠易激综合征（IBS）的发展有关，并且已经探索了各种方法来模拟与其他刺激相结合的IBS。然而,目前尚不清楚单独的应激是否能在动物中诱发IBS。本研究旨在研究慢性不可预测的轻度应激（CUMS）对小鼠胃肠道感觉和功能的影响，并评估CUMS作为IBS建模方法的潜力。为了评估老鼠的行为，我们进行了露天测试，蔗糖偏好测试和称重小鼠，表明CUMS确实引起小鼠的焦虑和抑郁，并导致体重减轻。进一步分析,包括粪便分析，全胃肠运输试验,和结肠推进测试，证明CUMS导致小鼠排便异常和胃肠动力中断。此外,腹部戒断反射试验表明CUMS暴露小鼠的内脏敏感性增加.使用苏木精和伊红染色进行的组织学检查显示，暴露于CUMS的小鼠结肠中没有明显的组织学改变，但确实有轻微的炎症细胞浸润.总之,研究结果表明，CUMS可以在小鼠体内复制IBS样症状，提供了一种新颖的自上而下的IBS建模方法。
