PDF(Pubmed)
Abstract：
UNASSIGNED: Within adipose tissue (AT), different macrophage subsets have been described, which played pivotal and specific roles in upholding tissue homeostasis under both physiological and pathological conditions. Nonetheless, studying resident macrophages in-vitro poses challenges, as the isolation process and the culture for extended periods can alter their inherent properties.
UNASSIGNED: Stroma-vascular cells isolated from murine subcutaneous AT were seeded on ultra-low adherent plates in the presence of macrophage colony-stimulating factor. After 4 days of culture, the cells spontaneously aggregate to form spheroids. A week later, macrophages begin to spread out of the spheroid and adhere to the culture plate.
UNASSIGNED: This innovative three-dimensional (3D) culture method enables the generation of functional mature macrophages that present distinct genic and phenotypic characteristics compared to bone marrow-derived macrophages. They also show specific metabolic activity and polarization in response to stimulation, but similar phagocytic capacity. Additionally, based on single-cell analysis, AT-macrophages generated in 3D culture mirror the phenotypic and functional traits of in-vivo AT resident macrophages.
UNASSIGNED: Our study describes a 3D in-vitro system for generating and culturing functional AT-resident macrophages, without the need for cell sorting. This system thus stands as a valuable resource for exploring the differentiation and function of AT-macrophages in vitro in diverse physiological and pathological contexts.
UNASSIGNED: Stroma-vascular cells isolated from murine subcutaneous AT were seeded on ultra-low adherent plates in the presence of macrophage colony-stimulating factor. After 4 days of culture, the cells spontaneously aggregate to form spheroids. A week later, macrophages begin to spread out of the spheroid and adhere to the culture plate.
UNASSIGNED: This innovative three-dimensional (3D) culture method enables the generation of functional mature macrophages that present distinct genic and phenotypic characteristics compared to bone marrow-derived macrophages. They also show specific metabolic activity and polarization in response to stimulation, but similar phagocytic capacity. Additionally, based on single-cell analysis, AT-macrophages generated in 3D culture mirror the phenotypic and functional traits of in-vivo AT resident macrophages.
UNASSIGNED: Our study describes a 3D in-vitro system for generating and culturing functional AT-resident macrophages, without the need for cell sorting. This system thus stands as a valuable resource for exploring the differentiation and function of AT-macrophages in vitro in diverse physiological and pathological contexts.
摘要：
■在脂肪组织（AT）内，已经描述了不同的巨噬细胞亚群，在生理和病理条件下，在维持组织稳态方面发挥了关键和特定的作用。尽管如此，在体外研究常驻巨噬细胞会带来挑战，因为隔离过程和长时间的培养可以改变它们的固有特性。
在巨噬细胞集落刺激因子存在下,将从鼠皮下AT分离的基质-血管细胞接种在超低粘附板上。培养4天后，细胞自发聚集形成球体。一周后,巨噬细胞开始从球体中扩散并粘附到培养板上。
■这种创新的三维（3D）培养方法能够产生功能性成熟的巨噬细胞，与骨髓来源的巨噬细胞相比，这些巨噬细胞具有不同的基因和表型特征。它们还显示出特定的代谢活性和对刺激的反应极化，但吞噬能力相似。此外,基于单细胞分析，在3D培养中产生的AT-巨噬细胞反映了体内AT常驻巨噬细胞的表型和功能特征。
■我们的研究描述了一种用于产生和培养功能性AT驻留巨噬细胞的3D体外系统，不需要细胞分选。因此，该系统是探索AT-巨噬细胞在不同生理和病理环境中体外分化和功能的宝贵资源。
在巨噬细胞集落刺激因子存在下,将从鼠皮下AT分离的基质-血管细胞接种在超低粘附板上。培养4天后，细胞自发聚集形成球体。一周后,巨噬细胞开始从球体中扩散并粘附到培养板上。
■这种创新的三维（3D）培养方法能够产生功能性成熟的巨噬细胞，与骨髓来源的巨噬细胞相比，这些巨噬细胞具有不同的基因和表型特征。它们还显示出特定的代谢活性和对刺激的反应极化，但吞噬能力相似。此外,基于单细胞分析，在3D培养中产生的AT-巨噬细胞反映了体内AT常驻巨噬细胞的表型和功能特征。
■我们的研究描述了一种用于产生和培养功能性AT驻留巨噬细胞的3D体外系统，不需要细胞分选。因此，该系统是探索AT-巨噬细胞在不同生理和病理环境中体外分化和功能的宝贵资源。
