PDF(Pubmed)
Abstract：
UNASSIGNED: Vital pulp therapy (VPT) is considered a conservative treatment for preserving pulp viability in caries and trauma-induced pulpitis. However, Mineral trioxide aggregate (MTA) as the most frequently used repair material, exhibits limited efficacy under inflammatory conditions. This study introduces an innovative nanocomposite hydrogel, tailored to simultaneously target anti-inflammation and dentin mineralization, aiming to efficiently preserve vital pulp tissue.
UNASSIGNED: The L-(CaP-ZnP)/SA nanocomposite hydrogel was designed by combining L-Arginine modified calcium phosphate/zinc phosphate nanoparticles (L-(CaP-ZnP) NPs) with sodium alginate (SA), and was characterized with TEM, SEM, FTIR, EDX, ICP-AES, and Zeta potential. In vitro, we evaluated the cytotoxicity and anti-inflammatory properties. Human dental pulp stem cells (hDPSCs) were cultured with lipopolysaccharide (LPS) to induce an inflammatory response, and the cell odontogenic differentiation was measured and possible signaling pathways were explored by alkaline phosphatase (ALP)/alizarin red S (ARS) staining, qRT-PCR, immunofluorescence staining, and Western blotting, respectively. In vivo, a pulpitis model was utilized to explore the potential of the L-(CaP-ZnP)/SA nanocomposite hydrogel in controlling pulp inflammation and enhancing dentin mineralization by Hematoxylin and eosin (HE) staining and immunohistochemistry staining.
UNASSIGNED: In vitro experiments revealed that the nanocomposite hydrogel was synthesized successfully and presented desirable biocompatibility. Under inflammatory conditions, compared to MTA, the L-(CaP-ZnP)/SA nanocomposite hydrogel demonstrated superior anti-inflammatory and pro-odontogenesis effects. Furthermore, the nanocomposite hydrogel significantly augmented p38 phosphorylation, implicating the involvement of the p38 signaling pathway in pulp repair. Significantly, in a rat pulpitis model, the L-(CaP-ZnP)/SA nanocomposite hydrogel downregulated inflammatory markers while upregulating mineralization-related markers, thereby stimulating the formation of robust reparative dentin.
UNASSIGNED: The L-(CaP-ZnP)/SA nanocomposite hydrogel with good biocompatibility efficiently promoted inflammation resolution and enhanced dentin mineralization by activating p38 signal pathway, as a pulp-capping material, offering a promising and advanced solution for treatment of pulpitis.
UNASSIGNED: The L-(CaP-ZnP)/SA nanocomposite hydrogel was designed by combining L-Arginine modified calcium phosphate/zinc phosphate nanoparticles (L-(CaP-ZnP) NPs) with sodium alginate (SA), and was characterized with TEM, SEM, FTIR, EDX, ICP-AES, and Zeta potential. In vitro, we evaluated the cytotoxicity and anti-inflammatory properties. Human dental pulp stem cells (hDPSCs) were cultured with lipopolysaccharide (LPS) to induce an inflammatory response, and the cell odontogenic differentiation was measured and possible signaling pathways were explored by alkaline phosphatase (ALP)/alizarin red S (ARS) staining, qRT-PCR, immunofluorescence staining, and Western blotting, respectively. In vivo, a pulpitis model was utilized to explore the potential of the L-(CaP-ZnP)/SA nanocomposite hydrogel in controlling pulp inflammation and enhancing dentin mineralization by Hematoxylin and eosin (HE) staining and immunohistochemistry staining.
UNASSIGNED: In vitro experiments revealed that the nanocomposite hydrogel was synthesized successfully and presented desirable biocompatibility. Under inflammatory conditions, compared to MTA, the L-(CaP-ZnP)/SA nanocomposite hydrogel demonstrated superior anti-inflammatory and pro-odontogenesis effects. Furthermore, the nanocomposite hydrogel significantly augmented p38 phosphorylation, implicating the involvement of the p38 signaling pathway in pulp repair. Significantly, in a rat pulpitis model, the L-(CaP-ZnP)/SA nanocomposite hydrogel downregulated inflammatory markers while upregulating mineralization-related markers, thereby stimulating the formation of robust reparative dentin.
UNASSIGNED: The L-(CaP-ZnP)/SA nanocomposite hydrogel with good biocompatibility efficiently promoted inflammation resolution and enhanced dentin mineralization by activating p38 signal pathway, as a pulp-capping material, offering a promising and advanced solution for treatment of pulpitis.
摘要：
■重要牙髓疗法（VPT）被认为是一种保守治疗方法，可在龋齿和外伤引起的牙髓炎中保持牙髓活力。然而,矿物三氧化物骨料(MTA)作为最常用的修补材料,在炎症条件下表现出有限的功效。这项研究介绍了一种创新的纳米复合水凝胶，同时针对抗炎和牙本质矿化，旨在有效地保存重要的牙髓组织。
■通过将L-精氨酸修饰的磷酸钙/磷酸锌纳米颗粒（L-（CaP-ZnP）NP）与海藻酸钠（SA）相结合，设计了L-（CaP-ZnP）/SA纳米复合水凝胶，并用TEM表征，SEM,FTIR,EDX,ICP-AES,和Zeta潜力。体外，我们评估了细胞毒性和抗炎特性。人牙髓干细胞（hDPSC）与脂多糖（LPS）一起培养以诱导炎症反应，并通过碱性磷酸酶（ALP）/茜素红S（ARS）染色检测细胞牙源性分化，探索可能的信号通路，qRT-PCR,免疫荧光染色，和西方印迹，分别。在体内,通过苏木精和伊红(HE)染色和免疫组织化学染色,利用牙髓炎模型探索L-(CaP-ZnP)/SA纳米复合水凝胶在控制牙髓炎症和增强牙本质矿化中的潜力.
■体外实验表明，成功合成了纳米复合水凝胶，并表现出理想的生物相容性。在炎症条件下，与MTA相比,L-(CaP-ZnP)/SA纳米复合水凝胶表现出优异的抗炎和促牙本质形成作用。此外，纳米复合水凝胶显着增强p38磷酸化，p38信号通路参与牙髓修复。重要的是,在大鼠牙髓炎模型中，L-（CaP-ZnP）/SA纳米复合水凝胶下调炎症标志物，同时上调矿化相关标志物，从而刺激形成强大的修复性牙本质。
■具有良好生物相容性的L-（CaP-ZnP）/SA纳米复合水凝胶通过激活p38信号通路有效促进炎症消退和增强牙本质矿化，作为纸浆封盖材料，为牙髓炎的治疗提供了一个有前途的和先进的解决方案。
■通过将L-精氨酸修饰的磷酸钙/磷酸锌纳米颗粒（L-（CaP-ZnP）NP）与海藻酸钠（SA）相结合，设计了L-（CaP-ZnP）/SA纳米复合水凝胶，并用TEM表征，SEM,FTIR,EDX,ICP-AES,和Zeta潜力。体外，我们评估了细胞毒性和抗炎特性。人牙髓干细胞（hDPSC）与脂多糖（LPS）一起培养以诱导炎症反应，并通过碱性磷酸酶（ALP）/茜素红S（ARS）染色检测细胞牙源性分化，探索可能的信号通路，qRT-PCR,免疫荧光染色，和西方印迹，分别。在体内,通过苏木精和伊红(HE)染色和免疫组织化学染色,利用牙髓炎模型探索L-(CaP-ZnP)/SA纳米复合水凝胶在控制牙髓炎症和增强牙本质矿化中的潜力.
■体外实验表明，成功合成了纳米复合水凝胶，并表现出理想的生物相容性。在炎症条件下，与MTA相比,L-(CaP-ZnP)/SA纳米复合水凝胶表现出优异的抗炎和促牙本质形成作用。此外，纳米复合水凝胶显着增强p38磷酸化，p38信号通路参与牙髓修复。重要的是,在大鼠牙髓炎模型中，L-（CaP-ZnP）/SA纳米复合水凝胶下调炎症标志物，同时上调矿化相关标志物，从而刺激形成强大的修复性牙本质。
■具有良好生物相容性的L-（CaP-ZnP）/SA纳米复合水凝胶通过激活p38信号通路有效促进炎症消退和增强牙本质矿化，作为纸浆封盖材料，为牙髓炎的治疗提供了一个有前途的和先进的解决方案。
