PDF(Pubmed)
Abstract：
Understanding the molecular and physical complexity of the tissue microenvironment (TiME) in the context of its spatiotemporal organization has remained an enduring challenge. Recent advances in engineering and data science are now promising the ability to study the structure, functions, and dynamics of the TiME in unprecedented detail; however, many advances still occur in silos that rarely integrate information to study the TiME in its full detail. This review provides an integrative overview of the engineering principles underlying chemical, optical, electrical, mechanical, and computational science to probe, sense, model, and fabricate the TiME. In individual sections, we first summarize the underlying principles, capabilities, and scope of emerging technologies, the breakthrough discoveries enabled by each technology and recent, promising innovations. We provide perspectives on the potential of these advances in answering critical questions about the TiME and its role in various disease and developmental processes. Finally, we present an integrative view that appreciates the major scientific and educational aspects in the study of the TiME.
摘要：
在时空组织的背景下理解组织微环境（TiME）的分子和物理复杂性仍然是一个持久的挑战。工程和数据科学的最新进展现在有望研究结构，功能,和TiME的动态以前所未有的细节；然而，许多进步仍然发生在筒仓中，这些筒仓很少整合信息来研究TiME的全部细节。这篇综述提供了化学基础工程原理的综合概述，光学，电气,机械,和计算科学来探索，感觉,模型，制造TiME。在个别章节中，我们首先总结了基本原理，能力,以及新兴技术的范围，每种技术和最近的突破性发现，有前途的创新。我们提供了这些进步在回答有关TiME及其在各种疾病和发育过程中的作用的关键问题方面的潜力的观点。最后,我们提出了一种综合观点，赞赏TiME研究中的主要科学和教育方面。
