UNASSIGNED: 120 patients with OSCC were included in this prospective cohort study. The levels of serum Chemerin were measured by enzyme-linked immunosorbent assay (ELISA). We also explored the possible effects of Chemerin on neutrophils\' chemokines in OSCC using a real-time PCR, western blotting.
UNASSIGNED: Levels of serum Chemerin, neutrophils and NLR were significantly higher among non-survivors compared to survivors of OSCC (both P < 0.05). Higher serum Chemerin levels were associated with advanced TNM stage, lymph node metastasis, differentiation and tumor recurrence (both P < 0.05). Serum Chemerin levels correlated with neutrophils and NLR levels (r = 0.708, r = 0.578, both P < 0.05). Based on ROC analysis, Chemerin + NLR predicted OSCC patient mortality with 81.54 % sensitivity and 87.27 % specificity, with an AUC of 0.8898. In a Kaplan-Meier analysis, high serum Chemerin levels, high neutrophil levels and high NLR levels were associated with shorter overall and disease-free survival (both P < 0.05). A univariate and multivariate Cox regression analysis showed that serum Chemerin and neutrophils were independent risk factors for OSCC. (both P < 0.05). QRT-PCR and western blotting results showed that Chemerin upregulated the expression of chemokines IL-17 and CXCL-5 in neutrophils (both P < 0.05).
UNASSIGNED: Our study suggests that measurement of serum Chemerin and neutrophils might be a useful diagnostic and prognostic biomarker for OSCC patients. Chemerin may promote neutrophils infiltration in OSCC through upregulation of chemokines IL17 and CXCL-5.
■120例OSCC患者纳入本前瞻性队列研究。采用酶联免疫吸附试验(ELISA)检测血清Chemerin水平。我们还使用实时PCR探索了Chemerin对OSCC中性粒细胞趋化因子的可能影响,西方印迹。
■血清Chemerin水平,与OSCC的幸存者相比,非幸存者的中性粒细胞和NLR显著升高(均P<0.05)。较高的血清Chemerin水平与晚期TNM分期相关,淋巴结转移,分化与肿瘤复发(均P<0.05)。血清Chemerin水平与中性粒细胞和NLR水平相关(r=0.708,r=0.578,均P<0.05)。基于ROC分析,Chemerin+NLR预测OSCC患者死亡率的敏感性为81.54%,特异性为87.27%。AUC为0.8898。在Kaplan-Meier分析中,高血清Chemerin水平,高中性粒细胞水平和高NLR水平与较短的总生存期和无病生存期相关(均P<0.05).单因素和多因素Cox回归分析显示血清Chemerin和中性粒细胞是OSCC的独立危险因素。(均P<0.05)。QRT-PCR和Westernblotting结果显示,Chemerin上调中性粒细胞中趋化因子IL-17和CXCL-5的表达(均P<0.05)。
■我们的研究表明,血清Chemerin和中性粒细胞的测量可能是OSCC患者的有用的诊断和预后生物标志物。Chemerin可通过上调趋化因子IL17和CXCL-5促进OSCC中的嗜中性粒细胞浸润。