PDF(Pubmed)
Abstract：
The potential presence of microplastics (MPs) in seafood products presents significant health concerns, demanding the adoption of standardized and validated methodologies. In this study, we introduce a validated method and an innovative technique for extracting MPs from mussels using an oxidizing agent, Corolase enzyme, and a surfactant, thus eliminating the need for mechanical agitation. Evaluation of the extraction process focused on three critical parameters: recovery percentage, repeatability, and chemical integrity, along with color stability. To ensure precision and reliability, low-density infrared spectroscopy (LDIR) was employed to analyze the effect of spectrum quality (Q). Ultimately, this methodology was applied to identify MPs in commercial mussels, with results showcasing the viability of the proposed validation stages for MPs extraction, maintaining MPs integrity with high recovery percentages.
摘要：
海鲜产品中潜在的微塑料（MP）存在严重的健康问题，要求采用标准化和经过验证的方法。在这项研究中,我们介绍了一种经过验证的方法和一种创新的技术，使用氧化剂从贻贝中提取MPs，Corolase酶，和表面活性剂，从而消除了机械搅拌的需要。对提取过程的评估集中在三个关键参数：回收率，重复性,和化学完整性，随着颜色的稳定性。为了确保精度和可靠性，采用低密度红外光谱（LDIR）分析了光谱质量（Q）的影响。最终,该方法用于识别商业贻贝中的MP，结果显示了拟议的MPs提取验证阶段的可行性，以很高的回收率保持国会议员的完整性。
