PDF(Pubmed)
Abstract：
The global application of the skin prick test (SPT) is attributed to the low costs, easy execution, and in vivo approach. Still, the healthcare professionals\' technique and the lancet shape may challenge the standardization of the method. Thus, we investigated the influence of the shape of the lancet and the applied weight on the wheal size of SPT. Two allergic and one non-allergic individual were tested with allergens (Dermatophagoides pteronyssinus and Phleum pratense) and histamine solution (positive control), respectively. Horizontally (HS) and diagonally (DS) shouldered lancets with the same tip length (1 mm) were tested under two different conditions: either 60 g or 120 g weight pressure. The wheal size induced by the 4 different combinations was measured. The higher-weight device (120 g) induced a significantly larger and less variable wheal response with the tested allergens and histamine. However, the shape of the lancet affected the wheal size more than the applied weight. The least variable response was measured to histamine for the horizontal-shouldered lancet combined with the higher weight, whereas the same lancet with the lower weight resulted in a significant number of false negative results.
摘要：
皮肤点刺试验（SPT）的全球应用归因于低成本，易于执行，和体内方法。尽管如此，医疗保健专业人员的技术和刺血针形状可能会挑战该方法的标准化。因此，我们研究了刺血针的形状和施加的重量对SPT的车轮尺寸的影响。两名过敏和一名非过敏个体用过敏原（翼状尘螨和口蹄疫）和组胺溶液（阳性对照）进行了测试，分别。在两种不同的条件下测试具有相同尖端长度(1mm)的水平(HS)和对角(DS)带肩刺血针:60g或120g重量压力。测量由4种不同组合诱导的水团大小。较高重量的装置（120g）对所测试的过敏原和组胺诱导了明显更大和更少的可变风团反应。然而,柳叶刀的形状比施加的重量更影响车轮尺寸。对于水平肩刺血针和较高重量的刺血针，测量到对组胺的变化最小的反应，而体重较低的相同刺血针会导致大量的假阴性结果。
