PDF(Pubmed)
Abstract：
UNASSIGNED: We aimed to contrast plasma amino acid concentrations in pregnant women with Gestational Diabetes Mellitus (GDM) to those without, to analyze the link between plasma amino acid concentrations, GDM, insulin resistance, and insulin secretion at 24-28 weeks of gestation.
UNASSIGNED: The research employed a retrospective case-control study design at a single center. Basic demographic and laboratory data were procured from the hospital\'s case system. The study encompassed seventy women without gestational diabetes mellitus (GDM) and thirty-five women with GDM matched in a 1-to-2 ratio for age and pre-pregnancy BMI. Utilizing high-performance liquid chromatography-mass spectrometry (HPLC-MS), peripheral fasting plasma amino acid concentrations in these women, during mid-pregnancy, were duly measured. We carefully evaluated the significant differences in the quantitative data between the two groups and developed linear regression models to assess the independent risk factors affecting insulin resistance and insulin secretion.
UNASSIGNED: Significant variations in insulin secretion and resistance levels distinguished GDM Group from the non-GDM group at three distinct time points, alongside relatively elevated serum Glycosylated Hemoglobin (HbA1c) levels. Triglycerides (TG) were also significantly increased in those with GDM during adipocytokine observations. Apart from glutamic acid and glutamine, the concentrations of the remaining 16 amino acids were notably increased in GDM patients, including all branched chain amino acids(BCAAs) and aromatic amino acids(AAAs). Ultimately, it was ascertained that fasting serum phenylalanine levels were independent risk factors affecting insulin resistance index and insulin secretion at various phases.
UNASSIGNED: Various fasting serum amino acid levels are markedly increased in patients with GDM, specifically phenylalanine, which may play role in insulin resistance and secretion.
UNASSIGNED: The research employed a retrospective case-control study design at a single center. Basic demographic and laboratory data were procured from the hospital\'s case system. The study encompassed seventy women without gestational diabetes mellitus (GDM) and thirty-five women with GDM matched in a 1-to-2 ratio for age and pre-pregnancy BMI. Utilizing high-performance liquid chromatography-mass spectrometry (HPLC-MS), peripheral fasting plasma amino acid concentrations in these women, during mid-pregnancy, were duly measured. We carefully evaluated the significant differences in the quantitative data between the two groups and developed linear regression models to assess the independent risk factors affecting insulin resistance and insulin secretion.
UNASSIGNED: Significant variations in insulin secretion and resistance levels distinguished GDM Group from the non-GDM group at three distinct time points, alongside relatively elevated serum Glycosylated Hemoglobin (HbA1c) levels. Triglycerides (TG) were also significantly increased in those with GDM during adipocytokine observations. Apart from glutamic acid and glutamine, the concentrations of the remaining 16 amino acids were notably increased in GDM patients, including all branched chain amino acids(BCAAs) and aromatic amino acids(AAAs). Ultimately, it was ascertained that fasting serum phenylalanine levels were independent risk factors affecting insulin resistance index and insulin secretion at various phases.
UNASSIGNED: Various fasting serum amino acid levels are markedly increased in patients with GDM, specifically phenylalanine, which may play role in insulin resistance and secretion.
摘要：
■我们旨在对比妊娠糖尿病（GDM）孕妇的血浆氨基酸浓度，分析血浆氨基酸浓度之间的联系，GDM,胰岛素抵抗，以及妊娠24-28周时的胰岛素分泌。
■本研究采用单中心回顾性病例对照研究设计。基本的人口统计学和实验室数据是从医院的病例系统获得的。该研究包括70名没有妊娠期糖尿病（GDM）的妇女和35名GDM妇女，年龄和孕前BMI为1：2。利用高效液相色谱-质谱(HPLC-MS),这些女性的外周空腹血浆氨基酸浓度，在怀孕中期,被适当地测量。我们仔细评估了两组之间定量数据的显着差异，并建立了线性回归模型来评估影响胰岛素抵抗和胰岛素分泌的独立危险因素。
■在三个不同的时间点，胰岛素分泌和抵抗水平的显着变化将GDM组与非GDM组区分开来。血清糖化血红蛋白（HbA1c）水平相对升高。在脂肪细胞因子观察期间，患有GDM的甘油三酸酯（TG）也显着增加。除了谷氨酸和谷氨酰胺,其余16种氨基酸的浓度在GDM患者中显著升高,包括所有支链氨基酸(BCAAs)和芳香族氨基酸(AAAs)。最终,确定空腹血清苯丙氨酸水平是影响不同时期胰岛素抵抗指数和胰岛素分泌的独立危险因素。
■GDM患者的各种空腹血清氨基酸水平明显升高，特别是苯丙氨酸，这可能在胰岛素抵抗和分泌中起作用。
■本研究采用单中心回顾性病例对照研究设计。基本的人口统计学和实验室数据是从医院的病例系统获得的。该研究包括70名没有妊娠期糖尿病（GDM）的妇女和35名GDM妇女，年龄和孕前BMI为1：2。利用高效液相色谱-质谱(HPLC-MS),这些女性的外周空腹血浆氨基酸浓度，在怀孕中期,被适当地测量。我们仔细评估了两组之间定量数据的显着差异，并建立了线性回归模型来评估影响胰岛素抵抗和胰岛素分泌的独立危险因素。
■在三个不同的时间点，胰岛素分泌和抵抗水平的显着变化将GDM组与非GDM组区分开来。血清糖化血红蛋白（HbA1c）水平相对升高。在脂肪细胞因子观察期间，患有GDM的甘油三酸酯（TG）也显着增加。除了谷氨酸和谷氨酰胺,其余16种氨基酸的浓度在GDM患者中显著升高,包括所有支链氨基酸(BCAAs)和芳香族氨基酸(AAAs)。最终,确定空腹血清苯丙氨酸水平是影响不同时期胰岛素抵抗指数和胰岛素分泌的独立危险因素。
■GDM患者的各种空腹血清氨基酸水平明显升高，特别是苯丙氨酸，这可能在胰岛素抵抗和分泌中起作用。
