UNASSIGNED: The effects of plumbagin on S. frugiperda larval development and the activities of two detoxification enzymes were initially examined. Next, transcriptomic changes in S. frugiperda after plumbagin treatment were investigated. Furthermore, RNA-seq results were validated by qPCR.
UNASSIGNED: Plumbagin exhibited a high larvicidal activity against the second and third instar larvae of S. frugiperda with 72 h LC50 of 0.573 and 2.676 mg/g, respectively. The activities of the two detoxification enzymes carboxylesterase and P450 were significantly increased after 1.5 mg/g plumbagin treatment. Furthermore, RNA-seq analysis provided a comprehensive overview of complex transcriptomic changes in S. frugiperda larvae in response to 1.5 mg/g plumbagin exposure, and revealed that plumbagin treatment led to aberrant expression of a large number of genes related to nutrient and energy metabolism, humoral immune response, insect cuticle protein, chitin-binding proteins, chitin synthesis and degradation, insect hormone, and xenobiotic detoxification. The qPCR results further validated the reproducibility and reliability of the transcriptomic data.
UNASSIGNED: Our findings provide a valuable insight into understanding the insecticidal mechanism of the phytochemical plumbagin.
■初步研究了白金对S.frugiperda幼虫发育的影响以及两种解毒酶的活性。接下来,研究了plumbagin治疗后S.frugiperda的转录组变化。此外,通过qPCR验证RNA-seq结果。
■Plumbagin对S.frugiperda的第二龄和第三龄幼虫表现出很高的杀幼虫活性,72小时LC50为0.573和2.676mg/g,分别。1.5mg/g李子苷处理后,两种解毒酶羧酸酯酶和P450的活性显着提高。此外,RNA-seq分析提供了对1.5mg/g大李子素暴露反应的frugiperda幼虫复杂转录组变化的全面概述。并揭示了白金处理导致与营养和能量代谢相关的大量基因的异常表达,体液免疫反应,昆虫角质层蛋白,几丁质结合蛋白,几丁质的合成和降解,昆虫激素,和异国情调的解毒。qPCR结果进一步验证了转录组数据的可重复性和可靠性。
■我们的发现为理解植物化学大白花素的杀虫机理提供了宝贵的见解。