PDF(Pubmed)
Abstract：
The huhu beetle (Prionoplus reticularis) is the largest endemic beetle found throughout Aotearoa New Zealand, and is characterised by feeding on wood during its larval stage. It has been hypothesised that its gut microbiome plays a fundamental role in the degradation of wood. To explore this idea we examined the fungal and bacterial community composition of huhu grubs\' frass, using amplicon sequencing. Grubs were reared on an exclusive diet of either a predominantly cellulose source (cotton) or lignocellulose source (pine) for 4 months; subsequently a diet switch was performed and the grubs were grown for another 4 months. The fungal community of cellulose-reared huhu grubs was abundant in potential cellulose degraders, contrasting with the community of lignocellulose-reared grubs, which showed abundant potential soft rot fungi, yeasts, and hemicellulose and cellulose degraders. Cellulose-reared grubs showed a less diverse fungal community, however, diet switch from cellulose to lignocellulose resulted in a change in community composition that showed grubs were still capable of utilising this substrate. Conversely, diet seemed to have a limited influence on huhu grub gut bacterial communities.
摘要：
huhu甲虫（Prionoplusreticularis）是在新西兰奥特罗阿发现的最大的特有甲虫，其特点是在幼体阶段以木材为食。据推测，其肠道微生物群在木材降解中起着基本作用。为了探索这个想法，我们研究了胡胡氏菌的真菌和细菌群落组成，使用扩增子测序。在主要是纤维素来源（棉花）或木质纤维素来源（松树）的独家饮食中饲养Grubs4个月；随后进行饮食转换，并将g再生长4个月。纤维素饲养的胡胡氏菌的真菌群落在潜在的纤维素降解物中很丰富，与木质纤维素饲养的g的群落形成对比，显示出丰富的潜在软腐病真菌，酵母,以及半纤维素和纤维素降解剂。纤维素饲养的g表现出不那么多样化的真菌群落，然而,从纤维素到木质纤维素的饮食转变导致群落组成发生变化，表明g仍然能够利用这种底物。相反，饮食似乎对huhugrub肠道细菌群落的影响有限。
