Thermotolerant plant growth-promoting bacteria enhance growth and nutrient uptake of lettuce under heat stress conditions by altering stomatal movement and chlorophyll fluorescence.

耐热植物促生长细菌通过改变气孔运动和叶绿素荧光来增强莴苣在热胁迫条件下的生长和养分吸收。

• 影响指数: 3.023
• 发表时间: 2024 Jun
• 来源期刊: Physiol Mol Biol Plants
• DOI: 10.1007/s12298-024-01470-5
• PMID: 38974362
• 文章类型: Journal Article
• 作者列表: Chan TH,Ariyawansa HA,Rho H

关键词: Heat-stress tolerance; Lettuce; Nutrient content; Photosystem II efficiency; Stomatal conductance; Transpiration

Abstract

This study investigates the effects of selected PGPB on lettuce growth performance under heat-stress conditions. Bacterial plant growth-promoting potentials have been characterized and identified successfully in ongoing studies. Based on in vitro plant growth-promoting potential, the top five bacteria were ranked and identified as Acinetobacter sp. GRB12, Bacillus sp. GFB04, Klebsiella sp. LFB06, Klebsiella sp. GRB10, and Klebsiella sp. GRB04. They were mixed to inoculate on lettuce (Lactuca sativa L.) in temperature-controlled greenhouses. Another in-vivo chamber experiment was conducted by using Bacillus sp. GFB04 and Klebsiella sp. GFB10. Plant physiological traits (chlorophyll fluorescence and transpiration) and nutrient contents were measured at harvest, along with growth, development, and yield component analyses. Uninoculated plants under heat-stress condition showed poor growth performance. In contrast, plants with PGPB inoculation showed improved growth under heat-stress conditions, as the uptake of nutrients was facilitated by the symbionts. Inoculation also improved lettuce photosystem II efficiency and decreased total water use under heat stress. In conclusion, the current study suggests that PGPB inoculation successfully enhances lettuce heat-tolerance. PGPB application could potentially help improve sustainable production of lettuce with less fertilization under increasing temperatures.
UNASSIGNED: The online version contains supplementary material available at 10.1007/s12298-024-01470-5.

摘要

本研究调查了热胁迫条件下选择的PGPB对莴苣生长性能的影响。在正在进行的研究中已经成功地表征和鉴定了细菌植物生长促进潜力。基于体外植物生长促进潜力，排名前五位的细菌为不动杆菌。GRB12，芽孢杆菌。GFB04，克雷伯菌属。LFB06，克雷伯菌属。GRB10和克雷伯菌属。GRB04.将它们混合以在温度控制的温室中接种生菜（LactucasativaL.）。通过使用芽孢杆菌属进行另一个体内室实验。GFB04和克雷伯菌属。GFB10.植物生理性状(叶绿素荧光和蒸腾作用)和养分含量进行了测定,随着成长,发展,和产量成分分析。在热胁迫条件下未接种的植物显示出较差的生长性能。相比之下,接种PGPB的植物在热胁迫条件下显示出改善的生长，因为共生体促进了营养物质的吸收。接种还提高了莴苣光系统II的效率，并减少了热胁迫下的总用水量。总之,目前的研究表明，接种PGPB成功地提高了莴苣的耐热性。PGPB的应用可能有助于在温度升高的情况下减少施肥，从而改善莴苣的可持续生产。
■在线版本包含补充材料，可在10.1007/s12298-024-01470-5获得。