PDF(Pubmed)
Abstract：
UNASSIGNED: Cyclophosphamide (CP) is one of the most effective immunosuppressive agents. To understand the mechanisms used by the CP and MSCs in the kidney, we investigated their effects on some pathways.
UNASSIGNED: 4 groups of female rats were used. GI: was the normal control group treated with saline solution. Groups G II, G III, and G IV were treated with CP. G I and G II groups were sacrificed on the fourth day after treatment., G III (Auto healing group) was left without treatment after the CP injection for six days. The G IV group was treated with MSCs on the fourth day after the CP injection. G III and G IV groups were sacrificed six days after treatment, and the kidney was removed and processed.
UNASSIGNED: CP induced up-regulation in CD14 and CD21 positive cells and caspase. Significant down-regulation of previous markers in groups III and IV. CP exerted a downregulation effect on AKT/ PI3K, that were ameliorated in groups III and IV. A significant increase in P53, BCL2, as well as VEGF in Group IV (P < 0 05).
UNASSIGNED: MSCs play a vital function in the immune inhibition in CP-treated rats through PI3K/AKT pathway.
UNASSIGNED: 4 groups of female rats were used. GI: was the normal control group treated with saline solution. Groups G II, G III, and G IV were treated with CP. G I and G II groups were sacrificed on the fourth day after treatment., G III (Auto healing group) was left without treatment after the CP injection for six days. The G IV group was treated with MSCs on the fourth day after the CP injection. G III and G IV groups were sacrificed six days after treatment, and the kidney was removed and processed.
UNASSIGNED: CP induced up-regulation in CD14 and CD21 positive cells and caspase. Significant down-regulation of previous markers in groups III and IV. CP exerted a downregulation effect on AKT/ PI3K, that were ameliorated in groups III and IV. A significant increase in P53, BCL2, as well as VEGF in Group IV (P < 0 05).
UNASSIGNED: MSCs play a vital function in the immune inhibition in CP-treated rats through PI3K/AKT pathway.
摘要：
■环磷酰胺（CP）是最有效的免疫抑制剂之一。为了了解CP和MSCs在肾脏中使用的机制，我们调查了它们对某些途径的影响。
■使用4组雌性大鼠。GI:是用盐水溶液处理的正常对照组。GII组，GIII,和GIV用CP治疗。GI组和GII组在治疗后第4天处死。,GIII（自动愈合组）在CP注射6天后不进行治疗。在CP注射后的第4天用MSCs处理GIV组。GIII和GIV组在治疗后6天处死，肾脏被切除和处理。
■CP诱导CD14和CD21阳性细胞以及胱天蛋白酶的上调。组III和IV中先前标志物的显著下调。CP对AKT/PI3K有下调作用，在III组和IV组中得到改善。IV组P53、BCL2和VEGF显著升高(P<005)。
■MSCs通过PI3K/AKT通路在CP处理大鼠的免疫抑制中发挥重要作用。
■使用4组雌性大鼠。GI:是用盐水溶液处理的正常对照组。GII组，GIII,和GIV用CP治疗。GI组和GII组在治疗后第4天处死。,GIII（自动愈合组）在CP注射6天后不进行治疗。在CP注射后的第4天用MSCs处理GIV组。GIII和GIV组在治疗后6天处死，肾脏被切除和处理。
■CP诱导CD14和CD21阳性细胞以及胱天蛋白酶的上调。组III和IV中先前标志物的显著下调。CP对AKT/PI3K有下调作用，在III组和IV组中得到改善。IV组P53、BCL2和VEGF显著升高(P<005)。
■MSCs通过PI3K/AKT通路在CP处理大鼠的免疫抑制中发挥重要作用。
