PDF(Pubmed)
Abstract：
Eggs not only contain all the molecules necessary to nurture new life but are also rich in nutrients such as high-quality protein. For example, epidemiologic studies have shown that egg intake is positively correlated with cognitive function. Thus, we specifically examined the effect of ovalbumin, a major protein present in egg whites, on cognitive function. First, we found that an orally administered enzymatic digest of ovalbumin improves cognitive function in mice fed a high-fat diet. Then, we narrowed down candidate peptides based on the prediction of peptide production according to enzyme-substrate specificity and comprehensive peptide analysis of the digest. We found that three peptides, namely ILPEY, LYRGGLEP, and ILELP, improve cognitive function after oral administration. We also showed that ILPEY, LYRGGLEP, and ILELP were present in the digest and named them ovomemolins A (OMA), B, and C, respectively. Notably, ovomemolins are the first peptides derived from egg whites that have been shown to improve cognitive function. The cognitive improvement induced by OMA, the most abundant of the peptides in the digest, was inhibited by methyllycaconitine, an antagonist of α7nAChR, which is known to be related to memory. These results suggest that OMA improves cognitive function through the acetylcholine system. After OMA administration, brain-derived neurotrophic factor (BDNF) mRNA expression and the number of 5-bromo-2\'-deoxyuridine-positive cells suggested that OMA increases hippocampal BDNF expression and neurogenesis.
摘要：
鸡蛋不仅含有培育新生命所必需的所有分子，而且还富含优质蛋白质等营养素。例如,流行病学研究表明，鸡蛋摄入量与认知功能呈正相关。因此，我们特别检查了卵清蛋白的作用，蛋白中的一种主要蛋白质，关于认知功能。首先,我们发现口服卵清蛋白酶消化可以改善高脂饮食小鼠的认知功能.然后，我们根据酶-底物特异性和消化的综合肽分析，根据肽产量的预测，缩小了候选肽的范围。我们发现三种肽，即ILPEY,LYRGGLEP,和ILELP,口服后改善认知功能。我们还展示了ILPEY,LYRGGLEP,和ILELP存在于消化中，并将其命名为ovomemolinsA（OMA），B,C,分别。值得注意的是,胚乳蛋白是由蛋白衍生的第一肽，已被证明可以改善认知功能。OMA诱导的认知改善，消化中最丰富的肽，被甲基云杉碱抑制，α7nAChR的拮抗剂,已知与记忆有关。这些结果表明OMA通过乙酰胆碱系统改善认知功能。OMA管理后，脑源性神经营养因子（BDNF）mRNA的表达和5-溴-2'-脱氧尿苷阳性细胞的数量表明OMA增加了海马BDNF的表达和神经发生。
