Abstract:
Recrystallization is considered the main damaging mechanism during the frozen storage of biologic materials. In this study, furcellaran, a polysaccharide related to κ-carrageenan, was studied for its concentration-dependent effect on ice crystal growth and recrystallization. The structure and sulfate content of the utilized furcellaran was analyzed by 1H nuclear magnetic resonance spectroscopy, ion chromatography, and high-performance size-exclusion chromatography. Additionally, the rheological properties of furcellaran solutions were investigated. Our findings demonstrate that furcellaran inhibits ice growth as effectively as κ-carrageenan. Furthermore, the rheological properties change with increasing furcellaran concentration, resulting in a gel-like consistency at 5 g/L, which coincides with decreased recrystallization inhibition activity and larger crystals. This suggests that gel formation or a gel-like consistency has to be avoided for optimal recrystallization inhibition activity.
摘要:
重结晶被认为是生物材料冷冻储存过程中的主要破坏机制。在这项研究中,furcellaran,一种与κ-角叉菜胶有关的多糖,研究了其对冰晶生长和重结晶的浓度依赖性影响。通过1H核磁共振波谱分析了所利用的furcellaran的结构和硫酸盐含量,离子色谱法,和高效尺寸排阻色谱法。此外,研究了furcellaran溶液的流变特性。我们的发现表明,furcellaran与κ-角叉菜胶一样有效地抑制冰的生长。此外,流变特性随着furcellaran浓度的增加而变化,导致5g/L的凝胶状稠度,这与重结晶抑制活性降低和较大的晶体一致。这表明必须避免凝胶形成或凝胶状稠度以获得最佳的重结晶抑制活性。