Abstract：
Secondary hemophagocytic lymphohistiocytosis (sHLH) is a hyperinflammatory syndrome characterized by immune disorders. It is imperative to elucidate the immunophenotypic panorama and the interactions among these cells in patients. Human peripheral blood mononuclear cells were collected from healthy donors and sHLH patients and tested using multicolor flow cytometry. We used FlowSOM to explore and visualize the immunophenotypic characteristics of sHLH. By demonstrating the phenotypes of immune cells, we discovered that sHLH patients had significantly higher levels of CD56+ monocytes, higher levels of myeloid-derived suppressor cells, low-density neutrophil-to-T cell ratio, and higher heterogeneous T cell activation than healthy donors. However, natural killer cell cytotoxicity and function were impaired. We then assessed the correlations among 30 immune cell types and evaluated metabolic analysis. Our findings demonstrated polymorphonuclear myeloid-derived suppressor cells, CD56+ monocytes, and neutrophil-to-T cell ratio were elevated abnormally in sHLH patients, which may indicate an association with immune overactivation and inflammatory response. We are expected to confirm that they are involved in the occurrence of the disease through further in-depth research.
摘要：
继发性噬血细胞性淋巴组织细胞增生症（sHLH）是一种以免疫疾病为特征的高炎症综合征。必须阐明患者的免疫表型全景和这些细胞之间的相互作用。从健康供体和sHLH患者收集人外周血单核细胞，并使用多色流式细胞术进行测试。我们使用FlowSOM探索和可视化sHLH的免疫表型特征。通过证明免疫细胞的表型，我们发现sHLH患者的CD56+单核细胞水平明显较高，更高水平的骨髓来源的抑制细胞，低密度中性粒细胞与T细胞的比率，和更高的异质性T细胞活化比健康供体。然而,自然杀伤细胞的细胞毒性和功能受损。然后，我们评估了30种免疫细胞类型之间的相关性并评估了代谢分析。我们的发现证明了多形核髓样来源的抑制细胞，CD56+单核细胞，sHLH患者的中性粒细胞与T细胞比率异常升高，这可能表明与免疫过度激活和炎症反应有关。我们有望通过进一步深入的研究证实他们参与了疾病的发生。
