Abstract：
The study aims to compare the action of Pleurotus cornucopiae and glibenclamide on alloxan-induced diabetes and ascertain how an aqueous extract of the edible mushroom regulates the expression of nuclear factor erythroid 2-related factor 2 (Nrf2), oxidative stress biomarkers and renal toxicity in a diabetic male Wistar rat model. Twenty-five adult male Wistar rats were randomly grouped into five groups with five rats per. Group 1 and those in the treatment groups received normal feed and water ad libitum. Group 2 received intraperitoneal administration of alloxan monohydrate (150 mg/kg body weight). Group 3 received alloxan monohydrate and glibenclamide (5 mg/kg body weight bwt), group 4 received alloxan monohydrate plus the extract (250 mg/kg bwt) and group 5 received alloxan monohydrate plus the extract (500 mg/kg bwt). The administration of glibenclamide plus the extract was oral for 14 days. Glibenclamide and the extract lowered blood glucose level, catalase, and glutathione peroxidase activities, increased the superoxide dismutase (SOD) activity in rats with alloxan induced diabetes. The extract at 500 mg/kg bwt reduced the plasma urea and sodium concentration in the treated rats. The extract and glibenclamide could detoxify alloxan and restore its induced renal degeneration and glomeruli atrophy, intra renal hemorrhage and inflammation and oxidative biomarkers through activation of Nrf2 expression. The drug glibenclamide and P. cornucopiae have appreciable hypoglycemic activity and potential to restore the normal renal architecture in the rats, hence they offer similar curative effects. Additionally, the extract at 500 mg/kg bwt activated SOD and Nrf2 expression more than glibenclamide in rats with alloxan-induced diabetes.
摘要：
该研究旨在比较杏鲍菇和格列本脲对四氧嘧啶诱导的糖尿病的作用，并确定食用菌的水提取物如何调节核因子类2相关因子2（Nrf2）的表达，糖尿病雄性Wistar大鼠模型中的氧化应激生物标志物和肾毒性。将25只成年雄性Wistar大鼠随机分为5组，每组5只大鼠。第1组和处理组中的那些免费接受正常饲料和水。第2组接受腹膜内施用四氧嘧啶一水合物(150mg/kg体重)。第3组接受四氧嘧啶一水合物和格列本脲（5mg/kg体重bwt），第4组接受四氧嘧啶一水合物加提取物（250mg/kgbwt），第5组接受四氧嘧啶一水合物加提取物（500mg/kgbwt）。格列本脲和提取物的给药是口服14天。格列本脲和提取物降低了血糖水平，过氧化氢酶,和谷胱甘肽过氧化物酶活性，增加了四氧嘧啶诱导的糖尿病大鼠的超氧化物歧化酶（SOD）活性。500mg/kgbwt的提取物降低了治疗大鼠的血浆尿素和钠浓度。提取物和格列本脲可以解毒四氧嘧啶并恢复其诱导的肾脏变性和肾小球萎缩，肾内出血和炎症和氧化生物标志物通过激活Nrf2表达。药物格列本脲和山茱萸具有明显的降血糖活性，并有可能恢复大鼠的正常肾脏结构，因此它们提供了相似的疗效。此外,在四氧嘧啶诱导的糖尿病大鼠中，500mg/kgbwt提取物激活的SOD和Nrf2表达高于格列本脲。
