Abstract：
The prevalence of metabolic disorders has been found to increase concomitantly with alternations in habitual diet and lifestyle, indicating the importance of metabolic health monitoring for early warning of high-risk status and suggesting effective intervention strategies. Hippuric acid (HA), as one of the most abundant metabolites from the gut microbiota, holds potential as a regulator of metabolic health. Accordingly, it is imperative to establish an efficient, sensitive, and affordable method for large-scale population monitoring, revealing the association between HA level and metabolic disorders. Upon systematic screening of macrocycle•dye reporter pair, a supramolecular architecture (guanidinomethyl-modified calix[5]arene, GMC5A) was employed to sense urinary HA by employing fluorescein (Fl), whose complexation behavior was demonstrated by theoretical calculations, accomplishing quantification of HA in urine from 249 volunteers in the range of 0.10 mM and 10.93 mM. Excitedly, by restricted cubic spline, urinary HA concentration was found to have a significantly negative correlation with the risk of metabolic disorders when it exceeded 0.76 mM, suggesting the importance of dietary habits, especially the consumption of fruits, coffee, and tea, which was unveiled from a simple questionnaire survey. In this study, we accomplished a high throughput and sensitive detection of urinary HA based on supramolecular sensing with the GMC5A•Fl reporter pair, which sheds light on the rapid quantification of urinary HA as an indicator of metabolic health status and early intervention by balancing the daily diet.
摘要：
代谢紊乱的患病率随着习惯性饮食和生活方式的改变而增加。指出代谢健康监测对高危状态预警的重要性，并提出有效的干预策略。马尿酸(HA),作为肠道微生物群最丰富的代谢产物之一，具有作为代谢健康调节剂的潜力。因此，必须建立一个有效的，敏感,和负担得起的大规模人口监测方法，揭示HA水平与代谢紊乱之间的关联。在系统筛选大环•染料报告子对后，超分子结构(胍基甲基修饰的杯[5]芳烃，GMC5A）通过使用荧光素（F1）来感测尿HA，其络合行为已通过理论计算证明，在0.10mM和10.93mM范围内完成249名志愿者尿液中HA的定量。兴奋,通过限制三次样条，当超过0.76mM时，发现尿HA浓度与代谢紊乱的风险显着负相关。表明饮食习惯的重要性，尤其是水果的消费，咖啡,茶，这是一个简单的问卷调查。在这项研究中,我们基于使用GMC5A•Fl报道分子对的超分子传感，实现了对尿HA的高通量和灵敏检测，通过平衡日常饮食，可以快速定量尿HA作为代谢健康状况的指标和早期干预。
