Abstract：
This research aimed to explore whether high-intensity focused ultrasound (HIFU) could conduct pulmonary artery denervation (PADN). HIFU was performed in pulmonary arteries of 6 normotensive rabbits at dose of 250W, 6 times for each rabbit, and an additional 6 rabbits served as controls. Then ATEPH was induced in both groups by intravenous infusion of autogeneic thrombus. Hemodynamics and ultrasonography parameters were measured by right heart catheter and echocardiography pre- and post-establishment of ATEPH models in both groups. Histological analysis and immunohistochemistry of tyrosine hydroxylase (TH) were also performed. After PADN procedures, 5 rabbits were successfully conducted PADN, of which ablation zone was also observed in right auricle or right lung in 4 rabbits. Ablation zone was detected only in right lung in 1 rabbit. Compared with control group, milder right heart hemodynamic changes were found in PADN group, accompanied by improved ultrasound parameters in PADN group. HIFU can acutly damage SNs around pulmonary artery successfully, which may be a new choice to conduct PADN. However, the accuracy of HIFU with PADN needs to be improved.
摘要：
本研究旨在探讨高强度聚焦超声（HIFU）是否可以进行肺动脉神经支配（PADN）。在6只血压正常的兔的肺动脉中进行HIFU，剂量为250W，每只兔子6次，另外6只兔子作为对照。然后通过静脉输注自体血栓在两组中诱导ATEPH。两组在ATEPH模型建立前后均通过右心导管和超声心动图测量血流动力学和超声参数。还进行了酪氨酸羟化酶（TH）的组织学分析和免疫组织化学。PADN程序后，5只兔子成功进行PADN，其中消融区也在4只兔子的右耳或右肺中观察到。1只兔仅在右肺中检测到消融区。与对照组相比,PADN组右心血流动力学变化较轻，PADN组超声参数改善。HIFU可以成功地严重损伤肺动脉周围的SNs，这可能是进行PADN的新选择。然而,PADN的HIFU精度有待提高。
