PDF(Pubmed)
Abstract：
Lactic acid bacteria (LAB) isolated from medicinal herb Murraya koenigii, commonly known as curry leaf, which promotes the growth and maintenance of gut microbiota, were studied for their probiotic potential. The key objective of this research was to isolate and evaluate probiotic characteristics, test adherence capabilities, and confirm their safety. Lactococcus lactis (MKL8), isolated from Murraya koenigii, was subjected to in vitro analysis to assess its resistance to the gastric environment, ability to adhere Caco-2 cells, anti-microbial activity, hydrophobicity, auto-aggregation, and safety profiling through MTT assay and hemolytic. MKL8 exhibited growth at 0.5% phenol concentrations (> 80%) and was able to survive in conditions with high bile concentrations (> 79%) and a relatively low pH (72%-91%). It shows high tolerance to high osmotic conditions (> 73%) and simulated gastric juice (> 72%). Additionally, MKL8 demonstrated strong hydrophobicity (85%), auto-aggregation (87.3%-91.7%), and adherence to Caco-2 cells. Moreover, it had an inhibitory effect against pathogens too. By performing the hemolytic and MTT assays, the non-toxicity of MKL8 isolate was examined, and it exhibited no harmful characteristics. Considering MKL8\'s resistance to gastrointestinal tract conditions, high surface hydrophobicity, non-toxicity, and ability to inhibit the tested pathogens, it can be concluded that MKL8 demonstrated promising probiotic properties and has potential for use in the food industry.
摘要：
从草药Murrayakoenigii中分离出的乳酸菌（LAB），通常被称为咖喱叶，促进肠道微生物群的生长和维持，研究了它们的益生菌潜力。这项研究的关键目标是分离和评估益生菌特性，测试坚持能力，确认他们的安全。乳酸乳球菌（MKL8），与Murrayakoenigii隔离，进行了体外分析，以评估其对胃环境的抵抗力，粘附Caco-2细胞的能力，抗微生物活性，疏水性,自动聚合，并通过MTT试验和溶血进行安全性分析。MKL8在0.5%苯酚浓度(>80%)下表现出生长,并且能够在具有高胆汁浓度(>79%)和相对低pH(72%-91%)的条件下存活。它显示出对高渗透条件(>73%)和模拟胃液(>72%)的高耐受性。此外,MKL8表现出较强的疏水性(85%),自动聚合（87.3%-91.7%），并粘附于Caco-2细胞。此外，它对病原体也有抑制作用。通过进行溶血和MTT测定，检查了MKL8分离株的无毒性，它没有表现出有害的特征。考虑到MKL8对胃肠道疾病的抵抗力，高表面疏水性，无毒性，以及抑制被测试病原体的能力，可以得出结论，MKL8显示出有希望的益生菌特性，并具有在食品工业中使用的潜力。
