Abstract：
The measurement of free hemoglobin (free Hb) in blood is crucial for assessing the risk of organ damage in patients with hemolytic diseases. However, the colorimetric method, commonly used in clinical practice, does not distinguish between free Hb and the hemoglobin-haptoglobin complex (Hb-Hp) in the blood, instead reflecting the total Hb level. Although size-exclusion high-performance liquid chromatography (SEC-HPLC) can specifically measure free Hb, its clinical use is limited by long assay times. Here, we developed a novel assay method for the rapid quantification of free Hb in serum, distinguishing it from Hb-Hp, using a latex agglutination immunoturbidimetric assay (LATIA). This method could be used to measure free Hb in sera in the range of 1-100 μg/mL in approximately 15 min using an automatic biochemistry analyzer. Using Hb-spiked serum samples from healthy adults, there was a high correlation with Hb levels determined using the newly developed method and SEC-HPLC, indicating a high specificity for free Hb. This novel assay can be used to monitor levels of free Hb in patients with various hemolytic diseases and to design therapeutic strategies based on measured values. However, further studies are required to assess its clinical performance.
摘要：
血液中游离血红蛋白（游离Hb）的测量对于评估溶血病患者器官损伤的风险至关重要。然而,比色法，在临床实践中常用，不区分游离Hb和血液中的血红蛋白-结合珠蛋白复合物（Hb-Hp），而不是反映总Hb水平。尽管尺寸排阻高效液相色谱（SEC-HPLC）可以特异性测量游离Hb，它的临床使用受到长的测定时间的限制。这里,我们开发了一种快速定量血清中游离Hb的新测定方法，区别于Hb-Hp,使用乳胶凝集免疫比浊法(LATIA)。该方法可用于使用自动生物化学分析仪在大约15分钟内测量血清中1至100μg/mL范围内的游离Hb。使用来自健康成年人的Hb加标血清样本，与使用新开发的方法和SEC-HPLC测定的Hb水平高度相关，表明对游离Hb的高特异性。这种新的测定法可用于监测患有各种溶血性疾病的患者中游离Hb的水平，并基于测量值设计治疗策略。然而,需要进一步的研究来评估其临床表现.
