Abstract：
Benzyl isothiocyanate (BITC) is a naturally active bacteriostatic substance and κ-carrageenan (KC) is a good film-forming substrate. In the present study, a nanoemulsion incorporating BITC was fabricated with a particle size of 224.1 nm and an encapsulation efficiency of 69.2 %. Subsequently, the acquired BITC nanoemulsion (BITC-NE) was incorporated into the KC-based film, and the light transmittance of the prepared composite films was lower than that of the pure KC film. Fourier transform infrared spectroscopy and scanning electron microscopy revealed that BITC-NE was compatible with the KC matrix. BITC-NE incorporation enhanced the tensile strength of the KC-based films by 33.7 %, decreased the elongation at break by 33.8 %, decreased the water vapor permeability by 60.1 %, increased the maximum thermal degradation temperature by 48.8 %, and decreased the oxygen permeability by 42 % (p < 0.05). Furthermore, the composite films showed enhanced antimicrobial activity against Staphylococcus aureus, Salmonella typhimurium, and Pseudomonas fluorescens. The developed KC-based composite films were applied to wrap raw beef, which significantly delayed the increase in total viable count, total volatile base nitrogen content, and thiobarbituric acid reactive substances, and prolonged the shelf-life of the raw beef by up to 10 days. These results indicated that the composite films prepared by incorporating BITC nanoemulsions into KC matrices have great antimicrobial application potential.
摘要：
异硫氰酸苄酯（BITC）是一种天然活性抑菌物质，κ-角叉菜胶（KC）是一种良好的成膜基质。在本研究中,制备包含BITC的纳米乳液,其具有224.1nm的粒度和69.2%的包封效率。随后，将获得的BITC纳米乳液（BITC-NE）掺入KC基膜中，制备的复合薄膜的透光率低于纯KC薄膜。傅里叶变换红外光谱和扫描电子显微镜显示BITC-NE与KC基质相容。BITC-NE的掺入使KC基薄膜的拉伸强度提高了33.7％，断裂伸长率降低了33.8%，水蒸气渗透率降低了60.1%，将最大热降解温度提高了48.8%，氧气渗透率降低了42%(p<0.05)。此外，复合膜对金黄色葡萄球菌显示出增强的抗菌活性，鼠伤寒沙门氏菌,和荧光假单胞菌.开发的KC基复合膜用于包裹生牛肉，这显著延迟了总可行数的增加，总挥发性碱氮含量，和硫代巴比妥酸活性物质，将生牛肉的保质期延长了10天。这些结果表明，通过将BITC纳米乳液掺入KC基质中制备的复合膜具有很大的抗微生物应用潜力。
