Abstract：
Three functional magnetic nanocatalytic probe, which integrates recognition, catalytic amplification, and separation enrichment, is a new approach to construct a simple, fast, highly selective, and sensitive analytical method. In this article, a new magnetic nanosurface molecularly imprinted polymer nanoprobe (Fe3O4@MIP) with trifunctionality was rapidly prepared using a microwave-assisted method with magnetic Fe3O4 nanoparticles as a substrate, chloramphenicol (CAP) as a template molecule, and methacrylic acid as a functional monomer. The characterized nanoprobe was found that could specifically recognize CAP, strongly catalyze the new indicator nanoreaction of fructose (DF)-HAuCl4. The gold nanoparticles (AuNPs) exhibit strong resonance Rayleigh scattering (RRS) and surface enhanced Raman scattering (SERS) effects. Upon addition of CAP, the SERS/RRS signals were linearly weakened. Accordingly, a new SERS/RRS analysis platform for highly sensitive and selective determination of CAP was constructed. The SERS linear range was 0.0125-0.1 nmol/L, with detection limit (DL) of 0.004 nmol/L CAP. Furthermore, it could be combined with magnet-enriched separation to further improve the sensitivity, with a DL of 0.04 pmol/L CAP. The SERS method has been used for the determination of CAP in real samples, with relative standard deviations of 2.37-9.89 % and the recovery of 95.24-107.1 %.
摘要：
三功能磁性纳米催化探针,集成了识别，催化放大，和分离富集，是一种新的方法来构建一个简单的，快,高度选择性,和灵敏的分析方法。在这篇文章中,以磁性Fe3O4纳米粒子为基底,采用微波辅助法快速制备了一种具有三官能度的磁性纳米表面分子印迹聚合物纳米探针(Fe3O4@MIP),氯霉素（CAP）作为模板分子，和甲基丙烯酸作为功能单体。发现表征的纳米探针可以特异性识别CAP，强催化果糖(DF)-HAuCl4的新指示剂纳米反应。金纳米粒子(AuNPs)表现出强共振瑞利散射(RRS)和表面增强拉曼散射(SERS)效应。添加CAP后，SERS/RRS信号呈线性减弱。因此，构建了一个新的SERS/RRS分析平台，用于高度灵敏和选择性地测定CAP。SERS线性范围为0.0125-0.1nmol/L,检出限(DL)为0.004nmol/LCAP。此外，它可以与富磁分离相结合，进一步提高灵敏度，DL为0.04pmol/LCAP。SERS方法已用于实际样品中CAP的测定，相对标准偏差为2.37-9.89%,回收率为95.24-107.1%。
