Abstract：
Cholestasis is a hepatic disease reported in humans, dogs, and chickens and is characterized by various signs. Bile duct ligation (BDL) is a standard model for research in cholestasis in male rats and mice. However, the timing and degree of structural changes in BDL-subjected liver differ in the two animal species. This study focused on chickens as a choice model for cholestasis. Specifically, we aimed to evaluate the features of BDL in hens and compare them with those in rats and mice. Eighteen hens, 19 female ICR mice, and 18 female SD rats were randomly divided into the sham-operated and BDL groups. At 2, 4, and 6 weeks after BDL, and 4 weeks after the sham operation, liver and blood samples were collected and analyzed histologically and biochemically. Histologically, bile duct proliferation in BDL-subjected livers was first observed in the chickens and then the rats and mice, whereas CD44-positive small hepatocytes were observed only in chickens in the BDL group. Biochemically, the mRNA expression of the hepatocyte growth factor was higher in BDL-subjected chickens, while Interleukin 6 expression was higher in the BDL-subjected rats and mice than in animals in the sham group. In addition, farnesoid X receptor mRNA expression was lower in the BDL-subjected chickens than in the sham chickens. The BDL group had significantly higher total bile acid blood concentration than the sham group. In conclusion, the signs of hepatopathy caused by BDL differ among animal species. Furthermore, we propose that compared to BDL-subjected mice and rats, BDL-subjected chickens are a novel cholestasis animal model that demonstrates severe hepatopathy and liver restructuring.
摘要：
胆汁淤积是人类报道的肝脏疾病，狗,和鸡，并以各种迹象为特征。胆管结扎（BDL）是雄性大鼠和小鼠胆汁淤积研究的标准模型。然而,在两种动物中，接受BDL的肝脏结构变化的时间和程度不同。本研究集中于鸡作为胆汁淤积的选择模型。具体来说,我们旨在评估母鸡BDL的特征，并将其与大鼠和小鼠的BDL进行比较。18只母鸡,19只雌性ICR小鼠，18只雌性SD大鼠随机分为假手术组和BDL组。在BDL后2、4和6周，假手术后4周，收集肝脏和血液样本,并进行组织学和生化分析.组织学上,首先在鸡中观察到BDL肝脏中的胆管增殖，然后在大鼠和小鼠中观察到，而CD44阳性的小肝细胞仅在BDL组中观察到。生物化学,肝细胞生长因子的mRNA表达在接受BDL的鸡中较高，而白细胞介素6在接受BDL的大鼠和小鼠中的表达高于假手术组的动物。此外,在接受BDL的鸡中，法尼醇X受体mRNA的表达低于假鸡。BDL组血总胆汁酸浓度明显高于假手术组。总之,由BDL引起的肝病的体征因动物物种而异。此外，我们认为，与BDL实验的小鼠和大鼠相比，受BDL影响的鸡是一种新型的胆汁淤积动物模型，表现出严重的肝病和肝重组。
