Abstract：
Powdery scab is an important potato disease caused by the soilborne pathogen Spongospora subterranea f. sp. subterranea. Currently, reliable chemical control and resistant cultivars for powdery scab are unavailable. As an alternative control strategy, we propose a novel approach involving the effective delivery of a phytocytokine to plant roots by the rhizobacterium Bacillus subtilis. The modified strain is designed to secrete the plant elicitor peptide StPep1. In our experiments employing a hairy root system, we observed a significant reduction in powdery scab pathogen infection when directly applying the StPep1 peptide. Furthermore, our pot assay, which involved pretreating potato roots with StPep1-secreting B. subtilis, demonstrated a substantial decrease in disease symptoms, including reduced root galling and fewer tuber skin scabs. These findings underscore the potential of engineered bacteria as a promising strategy for safeguarding plants against powdery scab.
摘要：
粉状sc病是由土壤传播的病原体Spongosporasubterraneaf引起的重要马铃薯病。sp。地下。目前,无法获得可靠的化学控制和抗粉状sc病的品种。作为一种替代控制策略，我们提出了一种新方法，涉及根瘤菌枯草芽孢杆菌将植物细胞因子有效递送到植物根部。修饰的菌株被设计为分泌植物诱导肽StPep1。在我们使用毛状根系统的实验中，当直接应用StPep1肽时，我们观察到粉状痂病原体感染显着减少。此外，我们的锅化验，其中涉及用分泌StPep1的枯草芽孢杆菌预处理马铃薯根，显示疾病症状大幅减少，包括减少根部磨损和减少块茎皮肤结痂。这些发现强调了工程化细菌作为保护植物免受粉状结痂的有希望的策略的潜力。
