PDF(Pubmed)
Abstract：
Isolating high-quality different cell types is a powerful approach for understanding cellular compositions and features in the heart, but it is challenging. The available protocols typically focus on isolating one or two cell types. Here, we present a protocol to simultaneously isolate high-quality and high-quantity cardiomyocytes and non-myocyte cells, including immune cells, from adult rat hearts. We describe steps for purifying cells using bovine serum albumin. We also detail procedures for viability analysis and cell type identification using fluorescence-activated cell sorting. For complete details on the use and execution of this protocol, please refer to Zhang et al.,1 Valkov et al.,2 Vang et al.,3 and Li et al.4.
摘要：
分离高质量的不同细胞类型是了解心脏细胞组成和特征的有效方法。但它具有挑战性。可用的方案通常集中于分离一种或两种细胞类型。这里,我们提出了同时分离高质量和大量心肌细胞和非肌细胞的协议，包括免疫细胞,从成年老鼠的心脏。我们描述了使用牛血清白蛋白纯化细胞的步骤。我们还详细介绍了使用荧光激活细胞分选进行活力分析和细胞类型鉴定的程序。有关此协议的使用和执行的完整详细信息，请参阅Zhang等人。,1Valkov等人。,2Vang等人。,3和李等4。
