Abstract：
Gold nanoclusters (AuNCs) possess weak intrinsic fluorescence, limiting their sensitivity in biosensing applications. This study addresses these limitations by developing a spatially confined dual-emission nanoprobe composed of silicon nanoparticles (SiNPs) and AuNCs. This amplified and stabilized fluorescence mechanism overcomes the limitations associated with using AuNCs alone, achieving superior sensitivity in the sensing platform. The nanoprobe was successfully employed for ratiometric detection of bleomycin (BLM) in serum samples, operating at an excitation wavelength of 365 nm, with emission wavelengths at 480 nm and 580 nm. The analytical performance of the system is distinguished by a linear detection range of 0-3.5 μM, an impressive limit of detection (LOD) of 35.27 nM, and exceptional recoveries ranging from 96.80 to 105.9%. This innovative approach significantly enhances the applicability and reliability of AuNC-based biosensing in complex biological media, highlighting its superior analytical capabilities.
摘要：
金纳米簇（AuNC）具有弱的固有荧光，限制了它们在生物传感应用中的灵敏度。本研究通过开发由硅纳米粒子（SiNPs）和AuNCs组成的空间受限双发射纳米探针来解决这些限制。这种放大和稳定的荧光机制克服了与单独使用AuNC相关的限制，在传感平台上实现卓越的灵敏度。纳米探针成功用于血清样品中博来霉素（BLM）的比率检测，在365nm的激发波长下工作，发射波长为480nm和580nm。系统的分析性能的特点是线性检测范围为0-3.5μM，令人印象深刻的检测限（LOD）35.27nM，以及96.80％至105.9％的特殊回收率。这种创新方法显着增强了基于AuNC的生物传感在复杂生物介质中的适用性和可靠性，突出其卓越的分析能力。
