Abstract：
The biological properties of human mesenchymal stromal cells (hMSCs) have been explored in over a thousand clinical trials in the last decade. Although hMSCs can be isolated from multiple sources, the degree of biological similarity between cell populations from these sources remains to be determined. A comparative study was performed investigating the growth kinetics and functionality of hMSCs isolated from adipose tissue (AT), bone marrow (BM) and umbilical cord tissue (UCT) expanded in monolayer over five passages. Adult hMSCs (AT, BM) had a slower proliferation ability than the UCT-hMSCs, with no apparent differences in their glucose consumption profile. BM-hMSCs produced higher concentrations of endogenous vascular endothelial growth factor (VEGF) compared to AT- and UCT-hMSCs. This study also revealed that UCT-hMSCs were more efficiently transduced by a lentiviral vector carrying a VEGF gene than their adult counterparts. Following cellular immunophenotypic characterization, no differences across the sources were found in the expression levels of the typical markers used to identify hMSCs. This work established a systematic approach for cell source selection depending on the hMSC\'s intended clinical application.
摘要：
在过去的十年中，人类间充质基质细胞（hMSC）的生物学特性已经在一千多个临床试验中得到了探索。虽然hMSCs可以从多个来源分离,来自这些来源的细胞群体之间的生物学相似性程度仍有待确定。进行了一项比较研究，研究了从脂肪组织（AT）分离的hMSCs的生长动力学和功能。骨髓（BM）和脐带组织（UCT）在五个通道中单层扩张。成年hMSCs（AT，BM）的增殖能力比UCT-hMSCs慢，他们的葡萄糖消耗曲线没有明显差异。与AT-和UCT-hMSC相比,BM-hMSC产生更高浓度的内源性血管内皮生长因子(VEGF)。该研究还揭示了UCT-hMSC被携带VEGF基因的慢病毒载体比它们的成人对应物更有效地转导。在细胞免疫表型表征之后，在用于鉴定hMSCs的典型标志物的表达水平上,没有发现不同来源的差异.这项工作根据hMSC的预期临床应用建立了系统的细胞来源选择方法。
