Abstract：
OBJECTIVE: In Brazil, urban arboviruses, such as dengue virus (DENV), Zika virus (ZIKV) and chikungunya virus (CHIKV), constitute a major public health problem, and due to their endemicity and asymptomatic cases, they pose a potential threat to blood donations. Rio de Janeiro (RJ), Brazil, has been impacted by extensive DENV epidemics over the last 30 years and, after 2015, by CHIKV and ZIKV.
METHODS: Urban arboviruses DENV, ZIKV and CHIKV were investigated in blood donations (n = 778) at the State Institute of Hematology, HEMORIO (RJ) from 2019 to 2022 by serological and molecular methods.
RESULTS: An overall arbovirus exposure was observed in 26.1% of the blood donations. Anti-DENV IgM was detected in 4.0% of samples and two donations were DENV NS1 positive. Positive anti-CHIKV IgM was observed in 4.7% of the donations. Co-detection of anti-CHIKV IgM and anti-DENV IgM was observed in 1.0% of donors, and CHIKV prevalence was 21.3%. All blood donations tested were negative for the DENV, ZIKV and CHIKV RNA.
CONCLUSIONS: IgM seroprevalence to the arboviruses analyzed here is an indicator of recent infection in asymptomatic donors, showing that the population of blood donors can be a vehicle for new infections, especially during epidemic periods.
METHODS: Urban arboviruses DENV, ZIKV and CHIKV were investigated in blood donations (n = 778) at the State Institute of Hematology, HEMORIO (RJ) from 2019 to 2022 by serological and molecular methods.
RESULTS: An overall arbovirus exposure was observed in 26.1% of the blood donations. Anti-DENV IgM was detected in 4.0% of samples and two donations were DENV NS1 positive. Positive anti-CHIKV IgM was observed in 4.7% of the donations. Co-detection of anti-CHIKV IgM and anti-DENV IgM was observed in 1.0% of donors, and CHIKV prevalence was 21.3%. All blood donations tested were negative for the DENV, ZIKV and CHIKV RNA.
CONCLUSIONS: IgM seroprevalence to the arboviruses analyzed here is an indicator of recent infection in asymptomatic donors, showing that the population of blood donors can be a vehicle for new infections, especially during epidemic periods.
摘要：
目标：在巴西，城市虫媒病毒，如登革热病毒（DENV），寨卡病毒（ZIKV）和基孔肯雅病毒（CHIKV），构成重大公共卫生问题，由于它们的地方性和无症状病例，它们对献血构成潜在威胁。里约热内卢(RJ),巴西,在过去的30年里，受到了广泛的DENV流行病的影响，2015年之后，CHIKV和ZIKV。
方法：城市虫媒病毒DENV，ZIKV和CHIKV在国家血液研究所进行了献血调查（n=778），通过血清学和分子方法，从2019年到2022年的HMORIO（RJ）。
结果：在26.1%的献血者中观察到总体虫媒病毒暴露。在4.0％的样品中检测到抗DENVIgM，两个捐赠为DENVNS1阳性。在4.7%的捐赠中观察到阳性抗CHIKVIgM。在1.0％的供体中观察到抗CHIKVIgM和抗DENVIgM的共检测，CHIKV患病率为21.3%。所有检测的献血都是DENV阴性，ZIKV和CHIKVRNA。
结论：本文分析的IgM对虫媒病毒的血清阳性率是无症状供体近期感染的指标，表明献血者可以成为新感染的载体，尤其是在流行期间。
方法：城市虫媒病毒DENV，ZIKV和CHIKV在国家血液研究所进行了献血调查（n=778），通过血清学和分子方法，从2019年到2022年的HMORIO（RJ）。
结果：在26.1%的献血者中观察到总体虫媒病毒暴露。在4.0％的样品中检测到抗DENVIgM，两个捐赠为DENVNS1阳性。在4.7%的捐赠中观察到阳性抗CHIKVIgM。在1.0％的供体中观察到抗CHIKVIgM和抗DENVIgM的共检测，CHIKV患病率为21.3%。所有检测的献血都是DENV阴性，ZIKV和CHIKVRNA。
结论：本文分析的IgM对虫媒病毒的血清阳性率是无症状供体近期感染的指标，表明献血者可以成为新感染的载体，尤其是在流行期间。
