METHODS: To determine the effect of autophagy on the LPS-induced inflammatory response, using various in vitro assays, we determined the effect of autophagy inhibitors and inducers on the inflammatory response in RAW264.7 cells.
RESULTS: Chloroquine (CQ), an autophagy inhibitor, suppressed pro-inflammatory cytokines, including interleukin (IL)-1β, IL-6, and tumor necrosis factor α (TNFα) in LPS-stimulated RAW264.7 cells. CQ also affected inflammatory mediators such as myeloid differentiation factor 88 and NF-kB in LPS-stimulated RAW264.7 cells.
CONCLUSIONS: This study demonstrated that CQ regulates the LPS-induced inflammatory response in RAW264.7 cells. We propose that targeting the regulation of pro-inflammatory cytokine levels and inflammatory mediators using CQ is a promising therapeutic approach for preventing inflammatory injury. CQ serves as a potential therapeutic target for treating various inflammatory diseases.
方法:为了确定自噬对LPS诱导的炎症反应的影响,使用各种体外试验,我们确定了自噬抑制剂和诱导剂对RAW264.7细胞炎症反应的影响.
结果:氯喹(CQ),自噬抑制剂,抑制促炎细胞因子,包括白细胞介素(IL)-1β,LPS刺激的RAW264.7细胞中的IL-6和肿瘤坏死因子α(TNFα)。CQ还影响LPS刺激的RAW264.7细胞中的炎症介质,如髓样分化因子88和NF-kB。
结论:本研究表明CQ调节LPS诱导的RAW264.7细胞炎症反应。我们建议使用CQ靶向调节促炎细胞因子水平和炎性介质是预防炎性损伤的有希望的治疗方法。CQ作为治疗各种炎性疾病的潜在治疗靶标。