Abstract：
Asthma is a widely prevalent chronic disease that brings great suffering to patients and may result in death if it turns severe. Jolkinolide B (JB) is one diterpenoid component separated from the dried roots of Euphorbia fischeriana Steud (Euphorbiaceae), and has anti--inflammatory, antioxidative, and antitumor properties. However, the detailed regulatory role and associated regulatory mechanism in the progression of asthma remain elusive. In this work, it was demonstrated that the extensive infiltration of bronchial inflammatory cells and the thickening of airway wall were observed in ovalbumin (OVA)-induced mice, but these impacts were reversed by JB (10 mg/kg) treatment, indicating that JB relieved the provocative symptoms in OVA-induced asthma mice. In addition, JB can control OVA-triggered lung function and pulmonary resistance. Moreover, JB attenuated OVA-evoked inflammation by lowering the levels of interleukin (IL)-4, IL-5, and IL-13. Besides, the activated nuclear factor kappa B (NF-κB) and transforming growth factor-beta-mothers against decapentaplegic homolog 3 (TGFβ/smad3) pathways in OVA-induced mice are rescued by JB treatment. In conclusion, it was disclosed that JB reduced allergic airway inflammation and airway remodeling in asthmatic mice by modulating the NF-κB and TGFβ/smad3 pathways. This work could offer new opinions on JB for lessening progression of asthma.
摘要：
哮喘是一种广泛流行的慢性疾病，给患者带来巨大的痛苦，如果变得严重，可能会导致死亡。JolkinolideB（JB）是一种二萜成分，从大的话科（大的话科）的干燥根中分离出来，有抗炎作用，抗氧化，和抗肿瘤特性。然而,在哮喘进展中的详细调控作用和相关调控机制仍然难以捉摸.在这项工作中,结果表明，在卵清蛋白（OVA）诱导的小鼠中观察到支气管炎症细胞的广泛浸润和气道壁的增厚，但这些影响被JB(10毫克/千克)治疗逆转，表明JB缓解了OVA诱导的哮喘小鼠的挑衅性症状。此外,JB可以控制OVA触发的肺功能和肺阻力。此外，JB通过降低白细胞介素（IL）-4，IL-5和IL-13的水平来减轻OVA诱发的炎症。此外,在OVA诱导的小鼠中，活化的核因子κB（NF-κB）和转化生长因子-β-母亲针对十一项截瘫同系物3（TGFβ/smad3）途径被JB治疗挽救。总之,据报道，JB通过调节NF-κB和TGFβ/smad3途径减少哮喘小鼠的过敏性气道炎症和气道重塑。这项工作可以为JB减轻哮喘的进展提供新的意见。
