PDF(Pubmed)
Abstract：
The gut microbiome of wild animals is subject to various environmental influences, including those associated with human-induced alterations to the environment. We investigated how the gut microbiota of a synurbic rodent species, the striped field mouse (Apodemus agrarius), change in cities of varying sizes, seeking the urban microbiota signature for this species. Fecal samples for analysis were collected from animals living in non-urbanized areas and green spaces of different-sized cities (Poland). Metagenomic 16S rRNA gene sequencing and further bioinformatics analyses were conducted. Significant differences in the composition of gut microbiomes among the studied populations were found. However, the observed changes were dependent on local habitat conditions, without strong evidence of a correlation with the size of the urbanized area. The results suggest that ecological detachment from a more natural, non-urban environment does not automatically lead to the development of an \"urban microbiome\" model in the studied rodent. The exposure to the natural environment in green spaces may serve as a catalyst for microbiome transformations, providing a previously underestimated contribution to the maintenance of native gut microbial communities in urban mammals.
摘要：
野生动物的肠道微生物群受各种环境影响，包括与人类引起的环境改变有关的那些。我们调查了共生啮齿动物的肠道微生物群，条纹田鼠（Apodemusagrarius），不同规模城市的变化，寻找这个物种的城市微生物群特征。用于分析的粪便样品是从生活在非城市化地区和不同大小城市（波兰）的绿地中的动物中收集的。进行了宏基因组16SrRNA基因测序和进一步的生物信息学分析。发现研究人群中肠道微生物组成存在显着差异。然而,观察到的变化取决于当地的栖息地条件，没有强有力的证据表明与城市化地区的大小相关。结果表明，生态脱离了更自然的，非城市环境不会自动导致研究的啮齿动物中“城市微生物组”模型的发展。在绿色空间中暴露于自然环境可以作为微生物组转化的催化剂，为维持城市哺乳动物的天然肠道微生物群落提供了以前被低估的贡献。
