Abstract：
Cold stress in low-temperature environments can trigger changes in gene expression, but epigenomics regulation of temperature stability in vital tissues, including the fat and diencephalon, is still unclear. Here, we explore the cold-induced changes in epigenomic features in the diencephalon and fat tissues of two cold-resistant Chinese pig breeds, Min and Enshi black (ES) pigs, utilizing H3K27ac CUT&Tag, RNA-seq, and selective signature analysis. Our results show significant alterations in H3K27ac modifications in the diencephalon of Min pigs and the fat of ES pigs after cold exposure. Dramatic changes in H3K27ac modifications in the diencephalon of Min pig are primarily associated with genes involved in energy metabolism and hormone regulation, whereas those in the fat of ES pig are primarily associated with immunity-related genes. Moreover, transcription factors PRDM1 and HSF1, which show evidence of selection, are enriched in genomic regions presenting cold-responsive alterations in H3K27ac modification in the Min pig diencephalon and ES pig fat, respectively. Our results indicate the diversity of epigenomic response mechanisms to cold exposure between Min and ES pigs, providing unique epigenetic resources for studies of low-temperature adaptation in large mammals.
摘要：
低温环境中的冷胁迫可以触发基因表达的变化，但是表观基因组学调节重要组织的温度稳定性，包括脂肪和间脑,还不清楚。这里,我们探讨了冷诱导的两个耐寒中国猪品种间脑和脂肪组织的表观基因组特征的变化，Min和恩施黑猪（ES），利用H3K27acCUT和标签，RNA-seq,和选择性特征分析。我们的结果表明，冷暴露后，Min猪的间脑和ES猪的脂肪中H3K27ac修饰发生了显着变化。Min猪H3K27ac修饰的巨大变化主要与涉及能量代谢和激素调节的基因有关，而ES猪的主要与免疫相关基因有关。此外，转录因子PRDM1和HSF1，它们显示了选择的证据，富集在Min猪间脑和ES猪脂肪中的H3K27ac修饰中呈现冷反应改变的基因组区域，分别。我们的结果表明，Min和ES猪对冷暴露的表观基因组反应机制的多样性，为大型哺乳动物低温适应研究提供了独特的表观遗传资源。
