Abstract：
The natural antimicrobial properties of essential oils (EOs) have contributed to the battle against multidrug-resistant microorganisms by providing new ways to develop more effective antibiotic agents. In this study, we investigated the chemical composition of Ocotea diospyrifolia essential oil (OdOE) and its antimicrobial properties combined with amikacin (AMK). Through gas chromatography-mass spectrometry (GCMS) analysis, the primary constituents of OdOE were identified as α-bisabolol (45.8%), β-bisabolene (9.4%), γ-elemene (7.6%), (Z)- β-farnesene (5.2%), spathulenol (3.5%), (Z)-caryophyllene (3.3%), and (E)-caryophyllene (3.1%). In vitro assessments showed that the combined administration of OdOE and AMK exerted a synergistic antibacterial effect on the multidrug-resistant K. pneumoniae strain. This synergistic effect demonstrated bacteriostatic action. OdEO combined with amikacin showed protein extravasation within 2 h of treatment, leading to bacterial death, which was determined by a reduction in viable cell count. The effective concentrations showed hemocompatibility. In vivo assessments using Caenorhabditis elegans as a model showed the survival of 85% of infected nematodes. Therefore, the combination OdEO combined with amikacin exhibited antimicrobial activity against a multidrug-resistant K. pneumoniae strain. Thus, OdOE is a promising agent that may be considered for development of antimicrobial treatment.
摘要：
精油（EO）的天然抗微生物特性通过提供新的方法来开发更有效的抗生素制剂，从而为对抗多药耐药微生物做出了贡献。在这项研究中,我们研究了Ocoteadiospyrifolia精油（OdOE）的化学成分及其与阿米卡星（AMK）结合的抗菌性能。通过气相色谱-质谱(GCMS)分析,OdOE的主要成分被鉴定为α-没药醇(45.8%)，β-双黑子烯（9.4%），γ-榄香烯（7.6%），(Z)-β-法尼烯(5.2%),spathulenol(3.5%),(Z)-石竹烯(3.3%),和(E)-石竹烯(3.1%)。体外评估显示,OdOE和AMK的组合施用对多重耐药性肺炎克雷伯菌菌株发挥协同抗菌作用。这种协同作用证明了抑菌作用。OdEO联合阿米卡星治疗2h内出现蛋白外渗，导致细菌死亡，这是通过活细胞计数的减少来确定的。有效浓度显示血液相容性。使用秀丽隐杆线虫作为模型的体内评估显示85%的感染线虫存活。因此,OdEO联合阿米卡星对多重耐药肺炎克雷伯菌菌株具有抗菌活性.因此，OdOE是一种有前途的药物，可以考虑用于开发抗微生物治疗。
