Abstract：
Iron deficiency remains a top nutrient deficiency worldwide. Iron chlorophyllin (IC), a compound structurally analogous to heme, utilizes the protoporphyrin ring of chlorophyll to bind iron. IC has previously been shown to deliver more iron to Caco-2 cells than FeSO4, the most common form prescribed for supplementation. However, previous test conditions used digestive conditions outside of those observed in humans. This study sought to assess IC bioaccessibility and Caco-2 cell uptake using physiologically relevant digestive solutions, pH, and incubation time, as compared to other iron sources (i.e. FeSO4, and hemoglobin (Hb)). Co-digestion with ascorbic acid (AA) and albumin was also investigated. Following gastric, duodenal, and jejunal digestion, IC-bound iron was less bioaccessible than iron delivered as FeSO4, and IC-bound iron was less bioaccessible than Hb-bound iron. IC-bound iron bioaccessibility was not affected by AA and was enhanced 2x with co-digested with a low dose of albumin. However, Caco-2 cell incubation with IC-containing digesta increased cell ferritin 2.5x more than FeSO4 alone, and less than Hb. IC with AA or with 400 mg albumin also increased cell ferritin more than IC alone, with the greatest increases observed following incubation of digesta containing IC + AA + 400 mg albumin. These results suggest IC can serve as an improved source of iron for supplementation as compared to FeSO4. These results also support further in vivo investigations of IC-based iron delivery in populations at risk of iron deficiency.
摘要：
缺铁仍然是全球最大的营养缺乏。叶绿素铁（IC），结构上类似于血红素的化合物，利用叶绿素的原卟啉环结合铁。先前已显示IC比FeSO4向Caco-2细胞递送更多的铁,FeSO4是规定用于补充的最常见形式。然而,以前的测试条件使用的是在人类中观察到的消化条件之外的消化条件。本研究旨在使用生理相关的消化液评估IC生物可及性和Caco-2细胞摄取。pH值,和孵化时间，与其他铁源(即FeSO4和血红蛋白(Hb))相比。还研究了与抗坏血酸(AA)和白蛋白的共消化。在胃病之后，十二指肠,和空肠消化，IC结合的铁的生物可及性低于以FeSO4形式递送的铁，IC结合的铁的生物可及性低于Hb结合的铁。IC结合的铁的生物可及性不受AA的影响，并且与低剂量的白蛋白共消化后可增强2x。然而,Caco-2细胞与含IC的消化物一起孵育，使细胞铁蛋白比单独的FeSO4增加2.5倍，低于Hb。含有AA或400毫克白蛋白的IC也比单独的IC增加更多的细胞铁蛋白，孵育含有ICAA400mg白蛋白的食糜后观察到最大的增加。这些结果表明，与FeSO4相比，IC可以作为补充铁的改进来源。这些结果还支持在有铁缺乏风险的人群中进行基于IC的铁递送的进一步体内研究。
