Abstract：
This study aims to investigate the alteration of salivary biomarker profiling in the development of oral submucous fibrosis (OSMF) and to explore the influence of saliva in the diagnosis of OSMF. A systematic search of published articles using the PRISMA guidelines was conducted to identify relevant studies on OSMF and saliva. All eligible studies, including case-control, cross-sectional studies, cohort, and pilot studies, contained the evaluation of salivary biomarker profiling in patients with OSMF. Salivary biomarker data from 28 selected articles were categorized into nine groups, and their mean values were determined. A three-step meta-analysis was performed by grouping salivary biomarker profiling into more heterogeneous categories based on OSMF classification, considering functional, histological, and clinical grading. The salivary biomarker profiling analysis revealed significant alterations in all markers, indicating their efficacy in OSMF diagnosis. Subgroup analyses highlighted significant associations in oxidative stress and protein with increased mean values, particularly emphasizing lipid peroxidase (LPO), malondialdehyde (MDA), and lactate dehydrogenase (LDH). Conversely, decreased mean values were observed in glutathione, glutathione peroxidase (GPx), superoxide dismutase (SOD), and vitamins. Notably, OSMF grading analysis demonstrated a significant difference in weighted effect sizes for histological grading, particularly in stage IV. The study underscores the alteration of specific salivary biomarkers, particularly those associated with LPO, MDA, LDH, glutathione, GPx, SOD, and vitamins, in diagnosing and grading OSMF.
摘要：
本研究旨在探讨口腔黏膜下纤维化（OSMF）发展过程中唾液生物标志物谱的变化，并探讨唾液在OSMF诊断中的影响。使用PRISMA指南对已发表的文章进行了系统搜索，以确定有关OSMF和唾液的相关研究。所有符合条件的研究，包括病例控制，横断面研究，队列,和试点研究，包含OSMF患者唾液生物标志物谱的评估。来自28篇选定文章的唾液生物标志物数据分为9组，并确定了它们的平均值。通过基于OSMF分类将唾液生物标志物分析分为更异质的类别，进行了三步荟萃分析。考虑到功能，组织学,和临床分级。唾液生物标志物谱分析揭示了所有标志物的显著改变，表明它们在OSMF诊断中的功效。亚组分析强调了氧化应激和蛋白质的显着关联，平均值增加，特别强调脂质过氧化物酶（LPO），丙二醛（MDA），和乳酸脱氢酶(LDH)。相反，在谷胱甘肽中观察到平均值降低，谷胱甘肽过氧化物酶（GPx），超氧化物歧化酶（SOD），和维生素。值得注意的是,OSMF分级分析表明，组织学分级的加权效应大小存在显着差异，特别是在第四阶段。该研究强调了特定唾液生物标志物的改变，特别是那些与LPO有关的，MDA,LDH,谷胱甘肽,GPx,SOD,和维生素,在OSMF的诊断和分级中。
