Abstract：
This study focused on detecting streptomycin (STR) residues using a luminescent aptasensor encapsulated with aptamer. Utilizing MOF-74-Co with peroxidase-like activity, luminol was enclosed in its pores. The specific STR aptamer acted as a gatekeeper, ensuring excellent performance. Upon exposure to STR, the aptamers detached, releasing luminol and amplifying the luminescent signal through MOF-74-Co catalytic activity. A linear relationship between fluorescence intensity and STR concentration (50 nM ∼ 5 × 106 nM) was established, with a limit of detection of 0.065 nM. The sensor exhibited high selectivity for STR even in the presence of other aminoglycoside antibiotics. Applied to tea, egg, and honey samples, the sensor showed recovery rates of 91.38-100.2%, meeting safety standards. This MOF-based aptasensor shows promise for detecting harmful residues.
摘要：
这项研究的重点是使用用适体封装的发光aptasensor检测链霉素（STR）残基。利用具有过氧化物酶样活性的MOF-74-Co，鲁米诺被封闭在它的孔中。特定的STR适体充当了看门人，确保优异的性能。暴露于STR后，适体分离，通过MOF-74-Co催化活性释放鲁米诺并放大发光信号。荧光强度与STR浓度(50nM~5×106nM)呈线性关系,检测限为0.065nM。即使在存在其他氨基糖苷类抗生素的情况下，该传感器也对STR表现出高选择性。适用于茶叶，鸡蛋,和蜂蜜样本,传感器的回收率为91.38-100.2%，符合安全标准。这种基于MOF的aptasensor有望检测有害残留物。
