Mesh : Beta vulgaris / metabolism Biodegradation, Environmental Oxygen / metabolism Aerobiosis Bioreactors / microbiology Biological Oxygen Demand Analysis Bacillus / metabolism Wastewater / microbiology chemistry Industrial Waste

来  源:   DOI:10.1371/journal.pone.0306330   PDF(Pubmed)

Abstract:
The efficiency of aerobic biodegradation of distillery wastewater using various microbial cultures is intricately linked to process conditions. The study aimed to examine the aerobic biodegradation by a Bacillus bacteria under controlled dissolved oxygen tension (DOT) conditions as a novel approach in the treatment of sugar beet distillery stillage. The processes were conducted in a 2-L Biostat®B stirred-tank reactor (STR), at a temperature of 36°C, with aeration of 1.0 L/(L·min), and uncontrolled pH of the medium (an initial pH of 8.0). Each experiment was performed at a different DOT setpoint: 75%, 65% and 55% saturation, controlled through stirrer rotational speed adjustments. The study showed that the DOT setpoint did not influence the process efficiency, determined by the pollutant load removal expressed as COD, BOD5 and TOC. In all three experiments, the obtained reduction values of these parameters were comparable, falling within the narrow ranges of 78.6-78.7%, 97.3-98.0% and 75.0-76.4%, respectively. However, the DOT setpoint did influence the rate of process biodegradation. The removal rate of the pollutant load expressed as COD, was the lowest when DOT was set at 55% (0.48 g O2/(L•h)), and the highest when DOT was set at 65% (0.55 g O2/(L•h)). For biogenic elements (nitrogen and phosphorus), a beneficial effect was observed at a low setpoint of controlled DOT during biodegradation. The maximum extent of removal of both total nitrogen (54%) and total phosphorus (67.8%) was achieved at the lowest DOT setpoint (55%). The findings suggest that conducting the batch aerobic process biodegradation of sugar beet stillage at a relatively low DOT setpoint in the medium might achieve high efficiency pollutant load removal and potentially lead to a reduction in the process cost.
摘要:
使用各种微生物培养物的酒厂废水的好氧生物降解效率与工艺条件密切相关。该研究旨在研究在受控的溶解氧张力(DOT)条件下芽孢杆菌的需氧生物降解,作为处理甜菜酒糟酒糟的新方法。该过程在2-LBiostat®B搅拌釜反应器(STR)中进行,在36°C的温度下,曝气为1.0L/(L·min),和培养基的不受控制的pH(8.0的初始pH)。每个实验在不同的DOT设定值下进行:75%,65%和55%饱和度,通过搅拌器转速调节控制。研究表明,DOT设定值不影响过程效率,由表示为COD的污染物负荷去除确定,BOD5和TOC。在所有三个实验中,获得的这些参数的还原值是可比的,落在78.6-78.7%的狭窄范围内,97.3-98.0%和75.0-76.4%,分别。然而,DOT设定值确实会影响过程生物降解的速率。以COD表示的污染物负荷的去除率,当DOT设定为55%(0.48gO2/(L·h))时,当DOT设定为65%(0.55gO2/(L·h))时最高。对于生物元素(氮和磷),在生物降解过程中,在受控DOT的低设定值下观察到有益效果。在最低DOT设定值(55%)下,可以最大程度地去除总氮(54%)和总磷(67.8%)。研究结果表明,在培养基中相对较低的DOT设定值下进行甜菜酒糟的分批好氧过程生物降解可能会实现高效的污染物负荷去除,并可能降低工艺成本。
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