PDF(Pubmed)
Abstract：
In recent years, the exploration of genome three-dimensional (3D) conformation has yielded profound insights into the regulation of gene expression and cellular functions in both animals and plants. While animals exhibit a characteristic genome topology defined by topologically associating domains (TADs), plants display similar features with a more diverse conformation across species. Employing advanced high-throughput sequencing and microscopy techniques, we investigated the landscape of 26 histone modifications and RNA polymerase II distribution in tomato (Solanum lycopersicum). Our study unveiled a rich and nuanced epigenetic landscape, shedding light on distinct chromatin states associated with heterochromatin formation and gene silencing. Moreover, we elucidated the intricate interplay between these chromatin states and the overall topology of the genome. Employing a genetic approach, we delved into the role of the histone modification H3K9ac in genome topology. Notably, our investigation revealed that the ectopic deposition of this chromatin mark triggered a reorganization of the 3D chromatin structure, defining different TAD-like borders. Our work emphasizes the critical role of H3K9ac in shaping the topology of the tomato genome, providing valuable insights into the epigenetic landscape of this agriculturally significant crop species.
摘要：
近年来,基因组三维（3D）构象的探索对动物和植物的基因表达和细胞功能的调节产生了深刻的见解。虽然动物表现出通过拓扑关联域（TAD）定义的特征性基因组拓扑，植物表现出相似的特征，物种之间的构象更加多样化。采用先进的高通量测序和显微镜技术，我们调查了番茄（Solanumlycopersicum）中26种组蛋白修饰和RNA聚合酶II分布的景观。我们的研究揭示了一个丰富而微妙的表观遗传景观，在与异染色质形成和基因沉默相关的不同染色质状态上发光。此外，我们阐明了这些染色质状态与基因组整体拓扑结构之间复杂的相互作用.采用遗传方法，我们探讨了组蛋白修饰H3K9ac在基因组拓扑结构中的作用。值得注意的是,我们的调查显示，这种染色质标记的异位沉积引发了3D染色质结构的重组，定义不同的类TAD边界。我们的工作强调了H3K9ac在塑造番茄基因组拓扑结构中的关键作用，为这种具有农业意义的作物物种的表观遗传景观提供有价值的见解。
