PDF(Pubmed)
Abstract：
BACKGROUND: Astragaloside IV is a main medicinal active ingredient in Astragalus membranaceus Bge. var. mongholicus (Bge.) Hsiao, which is also the key biomarker of A. membranaceus quality. Ethylene has been well-documented to involve in secondary metabolites biosynthesis in plants. Nevertheless, how ethylene regulates astragaloside IV biosynthesis in A. membranaceus is still unclear. Therefore, in the present study different dosages and time-dependent exogenous application of ethephon (Eth) were employed to analyze astragaloside IV accumulation and its biosynthesis genes expression level in hydroponically A. membranaceus.
RESULTS: Exogenous 200 µmol·L- 1Eth supply is most significantly increased astragaloside IV contents in A. membranaceus when compared with non-Eth supply. After 12 h 200 µmol·L- 1 Eth treatment, the astragaloside IV contents reaching the highest content at 3 d Eth treatment(P ≤ 0.05). Moreover, After Eth treatment, all detected key genes involved in astragaloside IV synthesis were significant decrease at 3rd day(P ≤ 0.05). However, SE displayed a significant increase at the 3rd day under Eth treatment(P ≤ 0.05). Under Eth treatment, the expression level of FPS, HMGR, IDI, SS, and CYP93E3 exhibited significant negative correlations with astragaloside IV content, while expression level of SE displayed a significant positive correlation.
CONCLUSIONS: These findings suggest that exogenous Eth treatment can influence the synthesis of astragaloside IV by regulating the expression of FPS, HMGR, IDI, SS, CYP93E3 and SE. This study provides a theoretical basis for utilizing molecular strategies to enhance the quality of A. membranaceus.
RESULTS: Exogenous 200 µmol·L- 1Eth supply is most significantly increased astragaloside IV contents in A. membranaceus when compared with non-Eth supply. After 12 h 200 µmol·L- 1 Eth treatment, the astragaloside IV contents reaching the highest content at 3 d Eth treatment(P ≤ 0.05). Moreover, After Eth treatment, all detected key genes involved in astragaloside IV synthesis were significant decrease at 3rd day(P ≤ 0.05). However, SE displayed a significant increase at the 3rd day under Eth treatment(P ≤ 0.05). Under Eth treatment, the expression level of FPS, HMGR, IDI, SS, and CYP93E3 exhibited significant negative correlations with astragaloside IV content, while expression level of SE displayed a significant positive correlation.
CONCLUSIONS: These findings suggest that exogenous Eth treatment can influence the synthesis of astragaloside IV by regulating the expression of FPS, HMGR, IDI, SS, CYP93E3 and SE. This study provides a theoretical basis for utilizing molecular strategies to enhance the quality of A. membranaceus.
摘要：
背景：黄芪甲苷是黄芪的主要药用活性成分。var.Mongholicus(Bge.)萧先生,这也是膜虫质量的关键生物标志物。乙烯已被充分证明参与植物的次生代谢产物的生物合成。然而，乙烯如何调节黄芪甲苷的生物合成尚不清楚。因此,在本研究中，采用不同剂量和时间依赖性外源应用乙烯利（Eth）来分析黄芪甲苷在水培A中的积累及其生物合成基因表达水平。
结果：与非Eth供应相比，外源200µmol·L-1Eth供应最显着增加了A。膜虫中黄芪甲苷IV的含量。经过12小时200µmol·L-1Eth处理，黄芪甲苷含量在3d处理时达到最高(P≤0.05)。此外，经过Eth治疗,所有参与黄芪甲苷合成的关键基因在第3天显著下降(P≤0.05)。然而,在Eth治疗的第3天，SE显着增加（P≤0.05）。在Eth治疗下,FPS的表达水平，HMGR,IDI,SS,CYP93E3与黄芪甲苷含量呈显著负相关,而SE的表达水平呈显著正相关。
结论：这些发现表明，外源Eth处理可以通过调节FPS的表达来影响黄芪甲苷的合成，HMGR,IDI,SS,CYP93E3和SE。本研究为利用分子策略提高膜虫质量提供了理论依据。
结果：与非Eth供应相比，外源200µmol·L-1Eth供应最显着增加了A。膜虫中黄芪甲苷IV的含量。经过12小时200µmol·L-1Eth处理，黄芪甲苷含量在3d处理时达到最高(P≤0.05)。此外，经过Eth治疗,所有参与黄芪甲苷合成的关键基因在第3天显著下降(P≤0.05)。然而,在Eth治疗的第3天，SE显着增加（P≤0.05）。在Eth治疗下,FPS的表达水平，HMGR,IDI,SS,CYP93E3与黄芪甲苷含量呈显著负相关,而SE的表达水平呈显著正相关。
结论：这些发现表明，外源Eth处理可以通过调节FPS的表达来影响黄芪甲苷的合成，HMGR,IDI,SS,CYP93E3和SE。本研究为利用分子策略提高膜虫质量提供了理论依据。
